This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Luber, P.
Right arrow Articles by Hahn, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Luber, P.
Right arrow Articles by Hahn, H.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, March 2003, p. 1062-1068, Vol. 41, No. 3
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.3.1062-1068.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Comparison of Broth Microdilution, E Test, and Agar Dilution Methods for Antibiotic Susceptibility Testing of Campylobacter jejuni and Campylobacter coli

Petra Luber,1* Edda Bartelt,1 Elke Genschow,1 Jutta Wagner,2 and Helmut Hahn2

Division of Food Hygiene, Federal Institute for Risk Assessment, D-12277 Berlin,1 Department of Medical Microbiology, Benjamin Franklin Medical Center, FU Berlin, D-12203 Berlin, Germany2

Received 22 April 2002/ Returned for modification 8 July 2002/ Accepted 8 October 2002

A standardized broth microdilution method was compared to the E test and an agar dilution method for the antimicrobial susceptibility testing of Campylobacter jejuni and C. coli isolates. A group of 47 human clinical isolates, 37 isolates from retail poultry, and 29 isolates from living turkeys (total, 113 isolates) was included in the study. These encompassed 92 C. jejuni and 21 C. coli strains. The MICs of six antimicrobial agents were determined by the broth microdilution and E test methods, and the strains of human origin were additionally tested by the agar dilution method. In general, broth microdilution MICs agreed within 1 log2 MIC increment with 90.0% of E test results and 78.7% of agar dilution test results. The agar dilution method gave much lower gentamicin MICs than the broth microdilution method, but the data were significantly (P < 0.01) correlated and there was 100% agreement in the sensitivities and specificities in the comparison of the tests. The broth microdilution method had the highest sensitivity for analysis of the susceptibilities of Campylobacter to nalidixic acid and trimethoprim-sulfamethoxazole. The MICs of ciprofloxacin and erythromycin complied numerically by all three methods. The classification of the results and the correlation of the data demonstrated a high degree of agreement. All methods were equally suitable for the testing of the sensitivity of Campylobacter to tetracycline. Thus, the broth microdilution method appears to be an easy and reliable method for determination of the MICs of antibiotics for C. jejuni and C. coli, and it may offer an interesting alternative to MIC determination by the agar dilution technique or the E test.

* Corresponding author. Mailing address: Division of Food Hygiene, Federal Institute for Risk Assessment, Diedersdorfer Weg 1, D-12277 Berlin, Germany. Phone: (0049-188) 8412-2135. Fax: (0049-188) 8412-2957. E-mail: p.luber{at}bfr.bund.de.

Journal of Clinical Microbiology, March 2003, p. 1062-1068, Vol. 41, No. 3
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.3.1062-1068.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Haas, M., Essig, A., Bartelt, E., Poppert, S. (2008). Detection of Resistance to Macrolides in Thermotolerant Campylobacter Species by Fluorescence In Situ Hybridization. J. Clin. Microbiol. 46: 3842-3844 [Abstract] [Full Text]  
  • Luangtongkum, T., Morishita, T. Y., El-Tayeb, A. B., Ison, A. J., Zhang, Q. (2007). Comparison of Antimicrobial Susceptibility Testing of Campylobacter spp. by the Agar Dilution and the Agar Disk Diffusion Methods. J. Clin. Microbiol. 45: 590-594 [Abstract] [Full Text]  
  • Vandenberg, O., Houf, K., Douat, N., Vlaes, L., Retore, P., Butzler, J.-P., Dediste, A. (2006). Antimicrobial susceptibility of clinical isolates of non-jejuni/coli campylobacters and arcobacters from Belgium. J Antimicrob Chemother 57: 908-913 [Abstract] [Full Text]  
  • Halbert, L. W., Kaneene, J. B., Mansfield, L. S., Ruegg, P. L., Warnick, L. D., Wells, S. J., Fossler, C. P., Campbell, A. M., Geiger-Zwald, A. M. (2005). Comparison of automated microbroth dilution and agar dilution for antimicrobial susceptibility of Campylobacter jejuni isolated from dairy sources. J Antimicrob Chemother 56: 686-691 [Abstract] [Full Text]  
  • Fitch, B. R., Sachen, K. L., Wilder, S. R., Burg, M. A., Lacher, D. W., Khalife, W. T., Whittam, T. S., Young, V. B. (2005). Genetic Diversity of Campylobacter sp. Isolates from Retail Chicken Products and Humans with Gastroenteritis in Central Michigan. J. Clin. Microbiol. 43: 4221-4224 [Abstract] [Full Text]  
  • Tu, Z.-C., Zeitlin, G., Gagner, J.-P., Keo, T., Hanna, B. A., Blaser, M. J. (2004). Campylobacter fetus of Reptile Origin as a Human Pathogen. J. Clin. Microbiol. 42: 4405-4407 [Abstract] [Full Text]  
  • Luber, P., Wagner, J., Hahn, H., Bartelt, E. (2003). Antimicrobial Resistance in Campylobacter jejuni and Campylobacter coli Strains Isolated in 1991 and 2001-2002 from Poultry and Humans in Berlin, Germany. Antimicrob. Agents Chemother. 47: 3825-3830 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»