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Journal of Clinical Microbiology, March 2003, p. 1069-1072, Vol. 41, No. 3
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.3.1069-1072.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Detection and Identification of Bartonella Species Pathogenic for Humans by PCR Amplification Targeting the Riboflavin Synthase Gene (ribC)

G. Johnson,1 M. Ayers,1 S. C. C. McClure,2 S. E. Richardson,1 and R. Tellier1*

Division of Microbiology, Hospital for Sick Children, University of Toronto,1 Cabbagetown Pet Clinic, Toronto, Ontario, Canada2

Received 30 September 2002/ Returned for modification 14 November 2002/ Accepted 5 December 2002

Several Bartonella species have now been implicated as human pathogens. The recovery of these fastidious organisms in the clinical microbiology laboratory remains difficult, and current methods are still relatively insensitive. Thus, the bartonellae are good candidates for detection by PCR. We have developed a PCR assay which uses a single primer pair targeting the riboflavin synthase gene (ribC) and detected six Bartonella species that have been implicated in human disease, B. henselae, B. quintana, B. bacilliformis, B. clarridgeiae, B. elizabethae, and B. vinsonii subsp. berkhoffii. Species identification is achieved simply by restriction enzyme digestion of the amplicon. This PCR assay appears to be specific for the Bartonella genus because it failed to amplify DNA from several other bacterial species.

* Corresponding author. Mailing address: Department of Paediatric Laboratory Medicine, The Hospital for Sick Children, 555 University Ave., Toronto, Ontario, Canada M5G 1X8. Phone: (416) 813-6592. Fax: (416) 813 6257. E-mail: raymond.tellier{at}sickkids.ca.

Journal of Clinical Microbiology, March 2003, p. 1069-1072, Vol. 41, No. 3
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.3.1069-1072.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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