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Journal of Clinical Microbiology, March 2003, p. 1135-1142, Vol. 41, No. 3
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.3.1135-1142.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Two-Center Collaborative Evaluation of the Performance of the BD Phoenix Automated Microbiology System for Identification and Antimicrobial Susceptibility Testing of Enterococcus spp. and Staphylococcus spp.

Anne-Marie Fahr,1* Ulrich Eigner,1 Martina Armbrust,1 Alexandra Caganic,1 Giuseppe Dettori,2 Carlo Chezzi,2 Luca Bertoncini,2 Magda Benecchi,2 and Maria Grazia Menozzi2

Department of Microbiology, Laboratory Group Heidelberg, D-69126 Heidelberg, Germany,1 Section of Microbiology, Department of Pathology and Laboratory Medicine, University of Parma, I-43100 Parma, Italy2

Received 9 August 2002/ Returned for modification 8 October 2002/ Accepted 15 December 2002

The performance of the BD Phoenix Automated Microbiology System (BD Diagnostic Systems, Sparks, Md.) was assessed for identification (ID) and antimicrobial susceptibility testing (AST) for the majority of clinically encountered bacterial isolates in a European collaborative two-center trial. A total of 469 bacterial isolates of the genera Staphylococcus (275 isolates), Enterococcus (179 isolates), and Streptococcus (15 isolates, for ID only) were investigated; of these, 367 were single patient isolates, and 102 were challenge strains tested at one center. Sixty-four antimicrobial drugs were tested, including the following drug classes: aminoglycosides, beta-lactam antibiotics, beta-lactam-beta-lactamase inhibitors, carbapenems, cephems, folate antagonists, quinolones, glycopeptides, macrolides-lincosamides-streptogramin B (MLS), and others. Phoenix ID results were compared to those of the laboratories' routine ID systems (API 32 Staph, API 32 Strep, and VITEK 2 [bioMérieux, Marcy l'Etoile, France]); Phoenix AST results were compared to those of frozen standard broth microdilution (SBM) panels according to NCCLS guidelines (NCCLS document M 100-S 9, approved standard M 7-A 4). Discrepant results were repeated in duplicate. Concordant IDs of 97.1, 98.9, and 100% were observed for staphylococci, enterococci, and streptococci, respectively. For AST results the overall essential agreement was 93.3%; the category agreement was 97.3%; and the very major error rate, major error rate, and minor error rate were 1.2, 1.9, and 1.3%, respectively. In conclusion, the Phoenix ID results showed high agreement with results of the systems to which they were being compared; the AST performance was highly equivalent to that of the SBM reference method.

* Corresponding author. Mailing address: Department of Microbiology, Laboratory Group Heidelberg, D-69126 Heidelberg, Germany. Phone: 49 6221 3432 125. Fax: 49 6221 3432 263. E-mail: A.Fahr{at}docnet.de.

Journal of Clinical Microbiology, March 2003, p. 1135-1142, Vol. 41, No. 3
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.3.1135-1142.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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