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Journal of Clinical Microbiology, March 2003, p. 1248-1251, Vol. 41, No. 3
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.3.1248-1251.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Entirely Automated Quantification of Human Immunodeficiency Virus Type 1 (HIV-1) RNA in Plasma by Using the Ultrasensitive COBAS AMPLICOR HIV-1 Monitor Test and RNA Purification on the MagNA Pure LC Instrument

Gabriele Hölzl, Markus Stöcher, Victoria Leb, Herbert Stekel, and Jörg Berg*

Institute of Laboratory Medicine, General Hospital Linz, A-4020 Linz, Austria

Received 15 July 2002/ Returned for modification 7 October 2002/ Accepted 20 December 2002

The ultrasensitive COBAS AMPLICOR HIV-1 Monitor test was complemented with automated RNA purification on the MagNA Pure LC instrument. This enabled entirely automated ultrasensitive assessment of viral loads in human immunodeficiency virus type 1 (HIV-1)-infected individuals. The detection limit of the fully automated assay and the viral load measurements in 80 clinical samples were found to be in good agreement with those of the conventional ultrasensitive COBAS AMPLICOR HIV-1 Monitor test. The fully automated assay showed markedly reduced hands-on time and was found to be suitable for the routine assessment of HIV-1 viral loads in a clinical diagnostic laboratory.

* Corresponding author. Mailing address: Institute of Laboratory Medicine, General Hospital Linz, Krankenhausstr. 9, A-4020 Linz, Austria. Phone: 43-70-7806-1174. Fax: 43-70-7806-1815. E-mail: joerg.berg{at}akh.linz.at.

Journal of Clinical Microbiology, March 2003, p. 1248-1251, Vol. 41, No. 3
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.3.1248-1251.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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