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Journal of Clinical Microbiology, March 2003, p. 1252-1255, Vol. 41, No. 3
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.3.1252-1255.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Novel Sample Preparation Method for Safe and Rapid Detection of Bacillus anthracis Spores in Environmental Powders and Nasal Swabs

Vicki A. Luna,1* Debra King,1 Carisa Davis,1 Tony Rycerz,1 Matthew Ewert,1 Andrew Cannons,1 Philip Amuso,1,2 and Jacqueline Cattani1

Center for Biological Defense, Department of Environmental and Occupational Health, College of Public Health, University of South Florida,1 Bureau of Laboratories, Florida Department of Health, Tampa, Florida 336122

Received 2 August 2002/ Returned for modification 16 November 2002/ Accepted 23 November 2002

Bacillus anthracis spores have been used as a biological weapon in the United States. We wanted to develop a safe, rapid method of sample preparation that provided safe DNA for the detection of spores in environmental and clinical specimens. Our method reproducibly detects B. anthracis in samples containing <10 spores.

* Corresponding author. Mailing address: Center for Biological Defense, University of South Florida, 3602 Spectrum Blvd., Tampa, FL 33612. Phone: (813) 974-3873. Fax: (813) 974-1479. E-mail: vluna{at}bt.usf.edu.

Journal of Clinical Microbiology, March 2003, p. 1252-1255, Vol. 41, No. 3
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.3.1252-1255.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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Antimicrob. Agents Chemother. Clin. Microbiol. Rev.
Clin. Vaccine Immunol. ALL ASM JOURNALS

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