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Journal of Clinical Microbiology, March 2003, p. 1270-1273, Vol. 41, No. 3
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.3.1270-1273.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Persistence of Colonization of Intestinal Mucosa by a Probiotic Strain, Lactobacillus casei subsp. rhamnosus Lcr35, after Oral Consumption

Christophe De Champs, Nathalie Maroncle, Damien Balestrino, Chantal Rich, and Christiane Forestier*

Laboratoire de Bactériologie, Facultés de Médecine-Pharmacie, Université d'Auvergne, Clermont-Ferrand, France

Received 23 September 2002/ Returned for modification 16 November 2002/ Accepted 5 December 2002

The colonization by the probiotic Lactobacillus casei subsp. rhamnosus Lcr35 of the gastrointestinal tracts of mice and humans was studied. The mice were orally given 109 CFU of Lcr35 either once or three times at 24-h intervals. A 16S ribosomal nucleic probe used in hybridization assays detected Lcr35 in the feces of mice for up to 3 days after the feeding, at a level of 108 to 109 CFU/g of feces. In the human assay, 12 healthy volunteers were enrolled in a randomized trial and ingested Lcr35 at a dosage of 108 or 1010 or 1012 CFU every day for 7 days. Then, after a 3-week posttreatment period, there was a second intake period similar to the first one. Analysis of fecal samples showed significant increases in the number of lactobacilli during the first intake period, whatever the dose given. The greatest increases were observed in subjects harboring the lowest indigenous population of Lcr35-like bacteria. During the 3-week posttreatment period, the number of CFU slightly decreased over time, and an increase, although not a statistically significant one, was observed during the second test period. These findings suggest that Lcr35 is able to survive within the gastrointestinal tract.

* Corresponding author. Mailing address: Laboratoire de Bactériologie, Université d'Auvergne, Faculté de Pharmacie, 28, place H. Dunant, 63000 Clermont-Ferrand, France. Phone: (33) 4 73 17 79 94. Fax: (33) 4 73 27 74 94. E-mail: Christiane.forestier{at}u-clermont1.fr.

Journal of Clinical Microbiology, March 2003, p. 1270-1273, Vol. 41, No. 3
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.3.1270-1273.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Coudeyras, S., Marchandin, H., Fajon, C., Forestier, C. (2008). Taxonomic and Strain-Specific Identification of the Probiotic Strain Lactobacillus rhamnosus 35 within the Lactobacillus casei Group. Appl. Environ. Microbiol. 74: 2679-2689 [Abstract] [Full Text]  
  • Ze-Ze, L., Tenreiro, R., Duarte, A., Salgado, M. J., Melo-Cristino, J., Lito, L., Carmo, M. M., Felisberto, S., Carmo, G. (2004). Case of aortic endocarditis caused by Lactobacillus casei. J Med Microbiol 53: 451-453 [Abstract] [Full Text]  


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