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Journal of Clinical Microbiology, March 2003, p. 993-997, Vol. 41, No. 3
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.3.993-997.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Rapid Detection of rpoB Gene Mutations in Rifampin-Resistant Mycobacterium tuberculosis Isolates in Shanghai by Using the Amplification Refractory Mutation System

Xiao-Yong Fan,1* Zhong-Yi Hu,2 Fan-Hong Xu,1 Zhi-Qiang Yan,3 Sheng-Qi Guo,1 and Zhong-Ming Li4

Molecular Biology Laboratory, Shanghai Institute of Biological Products,1 Tuberculosis Laboratory, Shanghai Lung Hospital,2 Shanghai Research Center of Biotechnology, Chinese Academy of Sciences, Shanghai, China ,3 Synergy America, Inc., Rockville, Maryland4

Received 8 August 2002/ Returned for modification 10 October 2002/ Accepted 12 December 2002

Rapid detection of drug resistance in Mycobacterium tuberculosis is essential for efficient treatment and control of this pathogen. The amplification refractory mutation system (ARMS) was used to detect mutations in the rifampin resistance-determining region of the rpoB gene. A total of 39 rifampin-resistant M. tuberculosis isolates in Shanghai were analyzed by this assay, resulting in 92.3% sensitivity (36 of 39) and 87.2% concordance (34 of 39) relative to DNA sequencing, by which 41 mutations of 11 different types, including 9 point mutations and 2 deletions, were identified in the rpoB gene. The most frequent mutations were those associated with codon 531 (21 of 39 [53.8%]) and codon 526 (9 of 39 [23.1%]). The results suggest that the ARMS assay is rapid and simple to implement and could be performed for detection of rifampin resistance in M. tuberculosis to complement conventional culture-based methods.

* Corresponding author. Mailing address: Molecular Biology Laboratory, Shanghai Institute of Biological Products, 1262, Yan'an Rd. (W), Shanghai, 200052, China. Phone: 86-21-62803189-62. Fax: 86-21-54191054. E-mail: xyfan008{at}hotmail.com.

Journal of Clinical Microbiology, March 2003, p. 993-997, Vol. 41, No. 3
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.3.993-997.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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