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Journal of Clinical Microbiology, April 2003, p. 1357-1362, Vol. 41, No. 4
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.4.1357-1362.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Use of Amplified Fragment Length Polymorphism Analysis To Identify Medically Important Candida spp., Including C. dubliniensis

A. Borst,1* B. Theelen,2 E. Reinders,3 T. Boekhout,2 A. C. Fluit,1 and P. H. M. Savelkoul3

Eijkman-Winkler Center for Microbiology, Infectious Diseases and Inflammation, University Medical Center,1 Centraalbureau voor Schimmelcultures, Utrecht,2 Department of Medical Microbiology and Infection Control, VU University Medical Center, Amsterdam, The Netherlands3

Received 22 July 2002/ Returned for modification 2 September 2002/ Accepted 22 December 2002

Non-Candida albicans Candida species are increasingly being isolated. These species show differences in levels of resistance to antimycotic agents and mortality. Therefore, it is important to be able to correctly identify the causative organism to the species level. Identification of C. dubliniensis in particular remains problematic due to the high degree of phenotypic similarity between this species and C. albicans. The use of amplified fragment length polymorphism (AFLP) analysis as an identification method for medically important Candida species was investigated. Our results show very clear differences among medically important Candida species. Furthermore, when screening a large collection of clinical isolates previously identified on CHROMagar as C. albicans, we found a misidentification rate of 6%. AFLP analysis is universally applicable, and the patterns can easily be stored in a general, accessible database. Therefore, AFLP might prove to be a reliable method for the identification of medically important Candida species.

* Corresponding author. Present address: Division of Bacterial and Mycotic Diseases, Centers for Disease Control and Prevention, Mailstop G-11, 1600 Clifton Rd., Atlanta, GA 30333. Phone: (404) 639-3547. Fax: (404) 639-3546. E-mail: aoz0{at}cdc.gov.

Journal of Clinical Microbiology, April 2003, p. 1357-1362, Vol. 41, No. 4
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.4.1357-1362.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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