JCM Figure table search 04
Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lindstedt, B.-A.
Right arrow Articles by Kapperud, G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lindstedt, B.-A.
Right arrow Articles by Kapperud, G.
Journal of Clinical Microbiology, April 2003, p. 1469-1479, Vol. 41, No. 4
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.4.1469-1479.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

DNA Fingerprinting of Salmonella enterica subsp. enterica Serovar Typhimurium with Emphasis on Phage Type DT104 Based on Variable Number of Tandem Repeat Loci

Bjørn-Arne Lindstedt,1* Even Heir,1 Elisabet Gjernes,1 and Georg Kapperud1,2

Division for Infectious Diseases Control, Norwegian Institute of Public Health, N-0403 Oslo,1 Department of Pharmacology, Microbiology, and Food Hygiene, Norwegian School of Veterinary Sciences, N-0033 Oslo, Norway2

Received 12 August 2002/ Returned for modification 16 September 2002/ Accepted 7 January 2003

Seventy-eight human and environmental strains of Salmonella enterica subsp. enterica serovar Typhimurium, as well as 18 isolates of other Salmonella serovars and 6 isolates of Escherichia coli, were subjected to a novel variable number of tandem repeats (VNTR)-based fingerprinting method that showed high discrimination and reproducibility for typing serovar Typhimurium isolates. The method is based on capillary separation of PCR products from fluorescence-labeled VNTR in the serovar Typhimurium genome. The serovar Typhimurium isolates displayed 54 VNTR patterns, and the VNTR assay correctly identified strains from a well-characterized outbreak. Among 37 serovar Typhimurium phage type DT104 isolates, 28 distinct VNTR patterns were found. This VNTR-based method is fast and suitable for complete automation. Our VNTR-based method was capable of high discrimination within the homogeneous serovar Typhimurium DT104 phage type and can be used to trace outbreaks and to monitor DT104 as well as other phage types. The VNTR assay was compared to XbaI pulsed-field gel electrophoresis, amplified fragment length polymorphism analysis, integron-cassette profiles and gene PCR of intI1, qacE{Delta}1, sulI1, and floR. The VNTR assay showed greatly improved resolution compared to all other tested methods in this study.

* Corresponding author. Mailing address: Division of Infectious Diseases Control, Norwegian Institute of Public Health, Geitmyrsveien 75, P.O. Box 4404, Nydalen, N-0403 Oslo, Norway. Phone: 47 22042200. Fax: 47 22042518. E-mail: bjorn-arne.lindstedt{at}fhi.no.

Journal of Clinical Microbiology, April 2003, p. 1469-1479, Vol. 41, No. 4
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.4.1469-1479.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:



Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
Antimicrob. Agents Chemother. Clin. Microbiol. Rev.
Clin. Vaccine Immunol. ALL ASM JOURNALS

Copyright © 2003 by the American Society for Microbiology. All rights reserved.