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Journal of Clinical Microbiology, April 2003, p. 1520-1524, Vol. 41, No. 4
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.4.1520-1524.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Drug-Susceptible Mycobacterium tuberculosis Beijing Genotype Does Not Develop Mutation-Conferred Resistance to Rifampin at an Elevated Rate

Jim Werngren* and Sven E. Hoffner

Department of Bacteriology, Swedish Institute for Infectious Disease Control, Solna, Sweden

Received 11 October 2002/ Returned for modification 23 December 2002/ Accepted 13 January 2003

The Mycobacterium tuberculosis Beijing genotype has drawn attention because it is often strongly associated with multidrug-resistant tuberculosis (MDR-TB). A possible reason is that the Beijing strains may have an enhanced capacity to develop drug resistance. In this study, we used the Luria-Delbrück fluctuation test to investigate whether strains of Beijing and non-Beijing genotypes exhibit differences in the acquisition of drug resistance. The M. tuberculosis reference strain H37Rv and 12 fully drug-susceptible clinical isolates, 6 of which were of the Beijing genotype, were examined. To determine the distribution of rifampin-resistant mutants, 25 independent cultures were made for each strain. The average mutation frequencies for the non-Beijing (H37Rv included) and Beijing genotypes were estimated to be 4.4 x 10-8 and 3.6 x 10-8, respectively. The corresponding average mutation rates for the non-Beijing and Beijing strains were 1.3 x 10-8 and 1.1x 10-8 mutations per cell division, respectively. The results suggest that the association of the Beijing genotype with MDR-TB is not due to an altered ability to develop resistance.

* Corresponding author. Mailing address: Department of Bacteriology, Swedish Institute for Infectious Disease Control (SMI), S-171 82 Solna, Sweden. Phone: 46-8-457 2443. Fax: 46-8-30 17 97. E-mail: jim.werngren{at}smi.ki.se.

Journal of Clinical Microbiology, April 2003, p. 1520-1524, Vol. 41, No. 4
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.4.1520-1524.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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