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Journal of Clinical Microbiology, April 2003, p. 1600-1608, Vol. 41, No. 4
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.4.1600-1608.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Detection of Ehrlichia spp., Anaplasma spp., Rickettsia spp., and Other Eubacteria in Ticks from the Thai-Myanmar Border and Vietnam

Philippe Parola,1,2,3* Jean-Paul Cornet,4 Yibayiri Osée Sanogo,2 R. Scott Miller,1 Huynh Van Thien,5 Jean-Paul Gonzalez,4 Didier Raoult,2 Sam R. Telford III,3 and Chansuda Wongsrichanalai1

Department of Immunology and Medicine, Armed Forces Research Institute of Medical Sciences, Bangkok,1 Institut de Recherche pour le Développement, UR34, Mahidol University at Salaya, Nakorn Prathom, Thailand,4 Unité des Rickettsies, CNRS UMR 6020, Faculté de Médecine, Marseille, France,2 Laboratory of Public Health Entomology, Harvard School of Public Health, Boston, Massachusetts,3 Bao Loc General Hospital, Bao Loc, Lam Dong Province, Vietnam5

Received 2 May 2002/ Returned for modification 6 November 2002/ Accepted 23 January 2003

A total of 650 ticks, including 13 species from five genera, were collected from animals, from people, or by flagging of the vegetation at sites on the Thai-Myanmar border and in Vietnam. They were tested by PCR to detect DNA of bacteria of the order Rickettsiales. Three Anaplasma spp. were detected in ticks collected in Thailand, including (i) Anaplasma sp. strain AnDa465, which was considered a genotype of Anaplasma platys (formerly Ehrlichia platys) and which was obtained from Dermacentor auratus ticks collected from dogs; (ii) Anaplasma sp. strain AnAj360, which was obtained from Amblyomma javanense ticks collected on a pangolin; and (iii) Anaplasma sp. strain AnHl446, which was closely related to Anaplasma bovis and which was detected in Haemaphysalis lagrangei ticks collected from a bear. Three Ehrlichia spp. were identified, including (i) Ehrlichia sp. strain EBm52, which was obtained from Boophilus microplus ticks collected from cattle from Thailand; (ii) Ehrlichia sp. strain EHh324, which was closely related to Ehrlichia chaffeensis and which was detected in Haemaphysalis hystricis ticks collected from wild pigs in Vietnam; and (iii) Ehrlichia sp. strain EHh317, which was closely related to Ehrlichia sp. strain EBm52 and which was also detected in H. hystricis ticks collected from wild pigs in Vietnam. Two Rickettsia spp. were detected in Thailand, including (i) Rickettsia sp. strain RDla420, which was detected in Dermacentor auratus ticks collected from a bear, and (ii) Rickettsia sp. strain RDla440, which was identified from two pools of Dermacentor larvae collected from a wild pig nest. Finally, two bacteria named Eubacterium sp. strain Hw124 and Eubacterium sp. strain Hw191 were identified in Haemaphysalis wellingtoni ticks collected from chicken in Thailand; these strains could belong to a new group of bacteria.

* Corresponding author. Mailing address: Unité des Rickettsies, CNRS UMR 6020, WHO Collaborative Center for Rickettsial Reference and Research, Faculté de Médecine, 27 Bd. Jean Moulin, 13385 Marseille Cedex 5, France. Phone: 33 4 91 32 43 75. Fax: 33 4 91 83 03 90. E-mail: phparola{at}yahoo.fr.

Journal of Clinical Microbiology, April 2003, p. 1600-1608, Vol. 41, No. 4
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.4.1600-1608.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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