This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Sieradzki, K.
Right arrow Articles by Tomasz, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sieradzki, K.
Right arrow Articles by Tomasz, A.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, April 2003, p. 1687-1693, Vol. 41, No. 4
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.4.1687-1693.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Evolution of a Vancomycin-Intermediate Staphylococcus aureus Strain In Vivo: Multiple Changes in the Antibiotic Resistance Phenotypes of a Single Lineage of Methicillin-Resistant S. aureus under the Impact of Antibiotics Administered for Chemotherapy

K. Sieradzki,1 T. Leski,1 J. Dick,2 L. Borio,2 and A. Tomasz1*

The Rockefeller University, New York, New York 10021,1 John Hopkins Medical Institutions, Baltimore, Maryland 212872

Received 2 October 2002/ Returned for modification 29 November 2002/ Accepted 7 January 2003

A number of methicillin-resistant Staphylococcus aureus (MRSA) isolates were recovered over a period of several weeks from blood samples and from the heart valve of a patient who underwent extensive vancomycin chemotherapy for persistent S. aureus bacteremia. Consecutive isolates showed gradually decreasing growth rates during in vitro cultivation and increasing vancomycin MICs, from an MIC of 1 µg/ml for the initial isolate to an MIC of 8 µg/ml for the final MRSA isolates, which also became tolerant to vancomycin. Major changes were observed in the oxacillin resistance phenotype of several of the isolates—apparently related to in vivo exposure to imipenem, which was also used during a period of chemotherapy. Both the gradually increasing vancomycin MICs and the changes in oxacillin resistance could be reproduced by appropriate exposure of the initial MRSA isolate to antibiotics in vitro. All isolates had the same pulsed-field gel electrophoresis pattern, spaA type, and multilocus sequence type (MLST), which was identified as a single-locus variant of ST5, the MLST characteristic of previously characterized MRSA isolates with reduced susceptibility to vancomycin in the United States and Japan.

* Corresponding author. Mailing address: The Rockefeller University, 1230 York Ave., New York, NY 10021. Phone: (212) 327-8277. Fax: (212) 327-8688. E-mail: tomasz{at}mail.rockefeller.edu.

Journal of Clinical Microbiology, April 2003, p. 1687-1693, Vol. 41, No. 4
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.4.1687-1693.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Mishra, N. N., Yang, S.-J., Sawa, A., Rubio, A., Nast, C. C., Yeaman, M. R., Bayer, A. S. (2009). Analysis of Cell Membrane Characteristics of In Vitro-Selected Daptomycin-Resistant Strains of Methicillin-Resistant Staphylococcus aureus. Antimicrob. Agents Chemother. 53: 2312-2318 [Abstract] [Full Text]  
  • Renzoni, A., Kelley, W. L., Barras, C., Monod, A., Huggler, E., Francois, P., Schrenzel, J., Studer, R., Vaudaux, P., Lew, D. P. (2009). Identification by Genomic and Genetic Analysis of Two New Genes Playing a Key Role in Intermediate Glycopeptide Resistance in Staphylococcus aureus. Antimicrob. Agents Chemother. 53: 903-911 [Abstract] [Full Text]  
  • Banik, J. J., Brady, S. F. (2008). Cloning and characterization of new glycopeptide gene clusters found in an environmental DNA megalibrary. Proc. Natl. Acad. Sci. USA 105: 17273-17277 [Abstract] [Full Text]  
  • Chung, M., Antignac, A., Kim, C., Tomasz, A. (2008). Comparative Study of the Susceptibilities of Major Epidemic Clones of Methicillin-Resistant Staphylococcus aureus to Oxacillin and to the New Broad-Spectrum Cephalosporin Ceftobiprole. Antimicrob. Agents Chemother. 52: 2709-2717 [Abstract] [Full Text]  
  • Olofsson, S. K., Marcusson, L. L., Stromback, A., Hughes, D., Cars, O. (2007). Dose-related selection of fluoroquinolone-resistant Escherichia coli. J Antimicrob Chemother 60: 795-801 [Abstract] [Full Text]  
  • Pereira, P. M., Filipe, S. R., Tomasz, A., Pinho, M. G. (2007). Fluorescence Ratio Imaging Microscopy Shows Decreased Access of Vancomycin to Cell Wall Synthetic Sites in Vancomycin-Resistant Staphylococcus aureus. Antimicrob. Agents Chemother. 51: 3627-3633 [Abstract] [Full Text]  
  • Fitzgibbon, M. M., Rossney, A. S., O'Connell, B. (2007). Investigation of Reduced Susceptibility to Glycopeptides among Methicillin-Resistant Staphylococcus aureus Isolates from Patients in Ireland and Evaluation of Agar Screening Methods for Detection of Heterogeneously Glycopeptide-Intermediate S. aureus. J. Clin. Microbiol. 45: 3263-3269 [Abstract] [Full Text]  
  • Mwangi, M. M., Wu, S. W., Zhou, Y., Sieradzki, K., de Lencastre, H., Richardson, P., Bruce, D., Rubin, E., Myers, E., Siggia, E. D., Tomasz, A. (2007). Tracking the in vivo evolution of multidrug resistance in Staphylococcus aureus by whole-genome sequencing. Proc. Natl. Acad. Sci. USA 104: 9451-9456 [Abstract] [Full Text]  
  • Wootton, M., MacGowan, A. P., Walsh, T. R., Howe, R. A. (2007). A Multicenter Study Evaluating the Current Strategies for Isolating Staphylococcus aureus Strains with Reduced Susceptibility to Glycopeptides. J. Clin. Microbiol. 45: 329-332 [Abstract] [Full Text]  
  • Howden, B. P., Johnson, P. D. R., Ward, P. B., Stinear, T. P., Davies, J. K. (2006). Isolates with Low-Level Vancomycin Resistance Associated with Persistent Methicillin-Resistant Staphylococcus aureus Bacteremia.. Antimicrob. Agents Chemother. 50: 3039-3047 [Abstract] [Full Text]  
  • Renzoni, A., Barras, C., Francois, P., Charbonnier, Y., Huggler, E., Garzoni, C., Kelley, W. L., Majcherczyk, P., Schrenzel, J., Lew, D. P., Vaudaux, P. (2006). Transcriptomic and Functional Analysis of an Autolysis-Deficient, Teicoplanin-Resistant Derivative of Methicillin-Resistant Staphylococcus aureus.. Antimicrob. Agents Chemother. 50: 3048-3061 [Abstract] [Full Text]  
  • McCallum, N., Karauzum, H., Getzmann, R., Bischoff, M., Majcherczyk, P., Berger-Bachi, B., Landmann, R. (2006). In Vivo Survival of Teicoplanin-Resistant Staphylococcus aureus and Fitness Cost of Teicoplanin Resistance.. Antimicrob. Agents Chemother. 50: 2352-2360 [Abstract] [Full Text]  
  • Sakoulas, G., Alder, J., Thauvin-Eliopoulos, C., Moellering, R. C. Jr., Eliopoulos, G. M. (2006). Induction of Daptomycin Heterogeneous Susceptibility in Staphylococcus aureus by Exposure to Vancomycin.. Antimicrob. Agents Chemother. 50: 1581-1585 [Abstract] [Full Text]  
  • McAleese, F., Wu, S. W., Sieradzki, K., Dunman, P., Murphy, E., Projan, S., Tomasz, A. (2006). Overexpression of Genes of the Cell Wall Stimulon in Clinical Isolates of Staphylococcus aureus Exhibiting Vancomycin-Intermediate- S. aureus-Type Resistance to Vancomycin. J. Bacteriol. 188: 1120-1133 [Abstract] [Full Text]  
  • Katayama, Y., Robinson, D. A., Enright, M. C., Chambers, H. F. (2005). Genetic Background Affects Stability of mecA in Staphylococcus aureus. J. Clin. Microbiol. 43: 2380-2383 [Abstract] [Full Text]  
  • Palazzo, I. C. V., Araujo, M. L. C., Darini, A. L. C. (2005). First Report of Vancomycin-Resistant Staphylococci Isolated from Healthy Carriers in Brazil. J. Clin. Microbiol. 43: 179-185 [Abstract] [Full Text]  
  • Renzoni, A., Francois, P., Li, D., Kelley, W. L., Lew, D. P., Vaudaux, P., Schrenzel, J. (2004). Modulation of Fibronectin Adhesins and Other Virulence Factors in a Teicoplanin-Resistant Derivative of Methicillin-Resistant Staphylococcus aureus. Antimicrob. Agents Chemother. 48: 2958-2965 [Abstract] [Full Text]  
  • Weiss, W. J., Murphy, T., Lenoy, E., Young, M. (2004). In Vivo Efficacy and Pharmacokinetics of AC98-6446, a Novel Cyclic Glycopeptide, in Experimental Infection Models. Antimicrob. Agents Chemother. 48: 1708-1712 [Abstract] [Full Text]  
  • Sieradzki, K., Tomasz, A. (2003). Alterations of Cell Wall Structure and Metabolism Accompany Reduced Susceptibility to Vancomycin in an Isogenic Series of Clinical Isolates of Staphylococcus aureus. J. Bacteriol. 185: 7103-7110 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»