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Journal of Clinical Microbiology, April 2003, p. 1694-1700, Vol. 41, No. 4
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.4.1694-1700.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Listeria monocytogenes Isolates from Invasive Infections: Variation of Sero- and Genotypes during an 11-Year Period in Finland

Susanna Lukinmaa,1 Maria Miettinen,2 Ulla-Maija Nakari,1 Hannu Korkeala,2 and Anja Siitonen1*

Laboratory of Enteric Pathogens, National Public Health Institute, FIN-00300 Helsinki,1 Department of Food and Environmental Hygiene, University of Helsinki, FIN-00014 Helsinki, Finland2

Received 27 September 2002/ Returned for modification 15 November 2002/ Accepted 7 January 2003

Listeria monocytogenes strains that were isolated from 314 human listeriosis cases in Finland during an 11-year period were analyzed by O:H serotyping and pulsed-field gel electrophoresis (PFGE). Serotyping divided the isolates into five serotypes, the most common being 1/2a (53%) and 4b (27%). During the study period, the number of cases caused by serotype 1/2a increased from 22% in 1990 to 67% in 2001, and those caused by serotype 4b decreased from 61 to 27%, respectively. PFGE with restriction enzyme AscI divided the strains into 81 PFGE genotypes; among strains of serotypes 1/2a and 4b, 49 and 18 PFGE types were seen, respectively. PFGE type 1 (serotype 1/2a) was the most prevalent single type (37 strains). Together with six other, closely related PFGE types, PFGE type 1 formed a group of 71 strains, representing 23% of all 314 strains. Strains of PFGE type 1 have also been isolated from cold smoked fish, suggesting a source of human infections caused by this type. Moreover, PFGE type 24 (serotype 1/2c) was significantly associated with gender: 5% of 180 male subjects but none of 132 female subjects (P = 0.012). An electronic database library was created from the PFGE profiles to make possible the prompt detection of new emerging profiles and the tracing of potential infection clusters in the future.

* Corresponding author. Mailing address: National Public Health Institute, Laboratory of Enteric Pathogens, Mannerheimintie 166, FIN-00300 Helsinki, Finland. Phone: 358-9-47448245. Fax: 358-9-47448238. E-mail: anja.siitonen{at}ktl.fi.

Journal of Clinical Microbiology, April 2003, p. 1694-1700, Vol. 41, No. 4
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.4.1694-1700.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Pan, Y., Breidt, F. Jr., Kathariou, S. (2009). Competition of Listeria monocytogenes Serotype 1/2a and 4b Strains in Mixed-Culture Biofilms. Appl. Environ. Microbiol. 75: 5846-5852 [Abstract] [Full Text]  
  • Lunden, J., Tolvanen, R., Korkeala, H. (2004). Human Listeriosis Outbreaks Linked to Dairy Products in Europe. J DAIRY SCI 87: E6-12 [Abstract] [Full Text]  


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