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Journal of Clinical Microbiology, April 2003, p. 1701-1704, Vol. 41, No. 4
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.4.1701-1704.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Detection of Hepatitis B Virus DNA in Sera from Patients with Chronic Hepatitis B Virus Infection by DNA Microarray Method

Kazunori Kawaguchi, Shuichi Kaneko,* Masao Honda, Hiroshi F. Kawai, Yukihiro Shirota, and Kenichi Kobayashi

Gastroenterology, Kanazawa University Graduate School of Medical Science, Takara-Machi 13-1, Kanazawa 920-8641, Japan

Received 3 June 2002/ Returned for modification 29 September 2002/ Accepted 14 December 2002

We have developed a sensitive and quantitative assay using a DNA microarray for the detection of hepatitis B virus (HBV) DNA in serum. Fluorescently labeled target cDNA prepared from cloned HBV DNA or serum HBV DNA was hybridized to capture DNA on a slide. A linear relationship was obtained between the intensities of the array spot and the amount of the cloned or serum HBV DNA, indicating the quantitative accuracy of this assay system. In addition, there was a significant correlation between the number of molecules of serum HBV DNA determined by the DNA microarray and that determined by a branched-DNA assay (n = 21, r = 0.89). Given these results, we conclude that the DNA microarray assay system may be useful as a diagnostic technique in the clinical laboratory.

* Corresponding author. Mailing address: Gastroenterology, Kanazawa University Graduate School of Medical Science, Takara-Machi 13-1, Kanazawa 920-8641, Japan. Phone: 81-76-265-2231. Fax: 81-76-234-4250. E-mail: skaneko{at}medf.m.kanazawa-u.ac.jp.

Journal of Clinical Microbiology, April 2003, p. 1701-1704, Vol. 41, No. 4
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.4.1701-1704.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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