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Journal of Clinical Microbiology, April 2003, p. 1710-1711, Vol. 41, No. 4
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.4.1710-1711.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Blood Agar and Mycobacterium tuberculosis: the End of a Dogma

M. Drancourt,1 P. Carrieri,2 M.-J. Gévaudan,1 and D. Raoult1*

Fédération de Microbiologie Clinique, Hôpital de la Timone, Assistance Publique—Hôpitaux de Marseille, IFR 48,1 INSERM U379, Marseille, France2

Received 26 July 2002/ Returned for modification 18 September 2002/ Accepted 20 October 2002

Incidental blood agar-based recovery of Mycobacterium tuberculosis led us to further investigate this routine medium for primary isolation and culture of M. tuberculosis. Fifteen respiratory tract and eight lymph node Ziehl-Neelsen-positive specimens were inoculated in parallel into tubes containing egg-based medium and 5% sheep blood agar. Colonies appeared sooner on this medium than on the egg-based medium, but this difference was not significant (P = 0.11, analysis of variance [ANOVA] test). Further experiments compared the growth of 38 respiratory and lymph node M. tuberculosis isolates when subcultured on the two media. After 6 days of incubation, 21 of 38 isolates had grown on blood agar, and the mean number of colonies was significantly greater on blood agar than on the egg-based medium (P < 0 0.001, ANOVA test). These results demonstrate that M. tuberculosis grows easily on blood agar within 1to 2 weeks, indicating that this basic medium is suitable for laboratory diagnosis of tuberculosis in addition to other media. Laboratories that routinely use prolonged incubations of blood plates, for example, for the recovery of Bartonella species, should consider the potential safety implications of encountering this highly infectious pathogen.

* Corresponding author. Mailing address: Unité des Rickettsies, Faculté de Médecine, 27 Boulevard Jean Moulin, 13385 Marseille cedex 5, France. Phone: 33 (0)4 91 32 43 75. Fax: 33 (0)4 91 83 03 90. E-mail: didier.raoult{at}medecine.univ-mrs.fr.

Journal of Clinical Microbiology, April 2003, p. 1710-1711, Vol. 41, No. 4
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.4.1710-1711.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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