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Journal of Clinical Microbiology, April 2003, p. 1726-1729, Vol. 41, No. 4
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.4.1726-1729.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Quantitative Detection of Chlamydia psittaci and C. pecorum by High-Sensitivity Real-Time PCR Reveals High Prevalence of Vaginal Infection in Cattle

Fred J. DeGraves,1 Dongya Gao,1 Hans-Robert Hehnen,2 Tobias Schlapp,2 and Bernhard Kaltenboeck1*

Department of Pathobiology, College of Veterinary Medicine, Auburn University, Auburn, Alabama 36849-5519,1 Bayer AG, Animal Health, Alfred-Nobel-Strasse 50, D-40789 Monheim, Germany2

Received 19 February 2002/ Returned for modification 6 October 2002/ Accepted 15 December 2002

Bovine vaginal cytobrush specimens were analyzed for the presence of Chlamydia spp. by a high-sensitivity, high-specificity quantitative PCR. The 53% prevalence of low-level Chlamydia psittaci and C. pecorum genital infection detected in virgin heifers suggests predominantely extragenital transmission of Chlamydia in cattle and conforms to the high seroprevalence of anti-Chlamydia antibodies.

* Corresponding author. Mailing address: Department of Pathobiology, College of Veterinary Medicine, Auburn University, 264 Greene Hall, Auburn, AL 36849-5519. Phone: 334-844-2665. Fax: 334-844-2652. E-mail: kaltebe{at}auburn.edu.

Journal of Clinical Microbiology, April 2003, p. 1726-1729, Vol. 41, No. 4
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.4.1726-1729.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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  • Menard, A., Clerc, M., Subtil, A., Megraud, F., Bebear, C., de Barbeyrac, B. (2006). Development of a real-time PCR for the detection of Chlamydia psittaci.. J Med Microbiol 55: 471-473 [Full Text]  
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