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Quantitative Detection of Chlamydia psittaci and C. pecorum by High-Sensitivity Real-Time PCR Reveals High Prevalence of Vaginal Infection in Cattle

Department of Pathobiology, College of Veterinary Medicine, Auburn University, Auburn, Alabama 36849-5519,¹ Bayer AG, Animal Health, Alfred-Nobel-Strasse 50, D-40789 Monheim, Germany²

Received 19 February 2002/ Returned for modification 6 October 2002/ Accepted 15 December 2002

Bovine vaginal cytobrush specimens were analyzed for the presence of Chlamydia spp. by a high-sensitivity, high-specificity quantitative PCR. The 53% prevalence of low-level Chlamydia psittaci and C. pecorum genital infection detected in virgin heifers suggests predominantely extragenital transmission of Chlamydia in cattle and conforms to the high seroprevalence of anti-Chlamydia antibodies.

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