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Journal of Clinical Microbiology, April 2003, p. 1736-1737, Vol. 41, No. 4
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.4.1736-1737.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Detection of Single Cells of Cryptococcus neoformans in Clinical Samples by Solid-Phase Cytometry

T. G. M. Bauters,1 D. Swinne,2 V. Stove,3 and H. J. Nelis1*

Laboratory for Pharmaceutical Microbiology, Department of Pharmaceutical Analysis, Ghent University,1 Department of Clinical Chemistry, Microbiology, and Immunology, Ghent University Hospital, Ghent,3 Department of Clinical Sciences, Institute of Tropical Medicine, Antwerp, Belgium2

Received 3 September 2002/ Returned for modification 7 October 2002/ Accepted 18 December 2002

A method based on solid-phase cytometry for the detection and enumeration of single cells of Cryptococcus neoformans in serum and cerebrospinal fluid is described. Both viable and nonviable cells are detected by using fluorescence viability labeling and immunofluorescence. This 30-min procedure has a detection limit of 3 to 6 cells per ml.

* Corresponding author. Mailing address: Laboratory for Pharmaceutical Microbiology, Harelbekestraat 72, B-9000 Ghent, Belgium. Phone: 32-9-264 80 91. Fax: 32-9-264 81 95. E-mail: Hans.Nelis{at}rug.ac.be.

Journal of Clinical Microbiology, April 2003, p. 1736-1737, Vol. 41, No. 4
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.4.1736-1737.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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