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Journal of Clinical Microbiology, April 2003, p. 1740-1742, Vol. 41, No. 4
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.4.1740-1742.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Enterobacterial Repetitive Intergenic Consensus 1R PCR Assay for Detection of Raoultella sp. Isolates among Strains Identified as Klebsiella oxytoca in the Clinical Laboratory

Sophie A. Granier,1,2 Véronique Leflon-Guibout,1,3 Fred W. Goldstein,2 and Marie-Hélène Nicolas-Chanoine1,3*

Service de Microbiologie-Hygiène, Hôpital Ambroise Paré AP-HP, Boulogne,1 Faculté Paris-Ile-de-France-Ouest, Université Versailles-Saint-Quentin-en-Yvelines, Versailles,3 Fondation Hôpital Saint-Joseph, Laboratoire de Microbiologie Médicale, Paris, France2

Received 16 September 2002/ Returned for modification 15 November 2002/ Accepted 10 January 2003

The enterobacterial repetitive intergenic consensus 1R PCR method, which provided recognizable profiles for reference strains of the three species of Raoultella and the two genetic groups of Klebsiella oxytoca, was applied to 19 clinical isolates identified as K. oxytoca. By this method, as confirmed by species-specific gene sequencing, two Raoultella ornithinolytica and two unclassifiable K. oxytoca isolates were identified.

* Corresponding author. Mailing address: Microbiology Department, Hôpital A. Paré, 9 avenue Charles de Gaulle, 92100 Boulogne-Billancourt, France. Phone: 33-1-49 09 55 40. Fax: 33-1-49 09 59 21. E-mail: marie-helene.nicolas-chanoine{at}apr.ap-hop-paris.fr.

Journal of Clinical Microbiology, April 2003, p. 1740-1742, Vol. 41, No. 4
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.4.1740-1742.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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