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Journal of Clinical Microbiology, April 2003, p. 1743-1746, Vol. 41, No. 4
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.4.1743-1746.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Use of Single-Enzyme Amplified Fragment Length Polymorphism for Typing Pasteurella multocida subsp. multocida Isolates from Pigs

A. M. Moreno,1* M. R. Baccaro,1 A. J. P. Ferreira,1 and A. F. Pestana de Castro2

Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo,1 Instituto de Ciências Biomédicas, Universidade de São Paulo, 05508-000 São Paulo, Sao Paulo, Brazil2

Received 4 September 2002/ Returned for modification 31 October 2002/ Accepted 7 January 2003

Single-enzyme amplified fragment length polymorphism (SE-AFLP) analyses were used to differentiate 97 isolates of porcine Pasteurella multocida subsp. multocida. The strains, isolated from animals with pneumonia, rhinitis, and septicemia, were classified as capsular types A, D, and F. SE-AFLP showed a discriminatory index of 0.87 and identified 18 different profiles.

* Corresponding author. Mailing address: Laboratório de Patologia Suína, Faculdade de Medicina Veterinária e Zootecnia-Universidade de São Paulo, Av. Prof. Dr. Orlando Marques de Paiva, n. 87, Cidade Universitária, 05508-000 São Paulo, SP, Brazil. Phone: 55 11 3091-1294. Fax: 55 11 3091-7829. E-mail: morenoam{at}uol.com.br.

Journal of Clinical Microbiology, April 2003, p. 1743-1746, Vol. 41, No. 4
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.4.1743-1746.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.





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