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Journal of Clinical Microbiology, April 2003, p. 1750-1752, Vol. 41, No. 4
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.4.1750-1752.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

New PCR Test That Recognizes All Human Prototypes of Enterovirus: Application for Clinical Diagnosis

Thomas Bourlet,1 Valerie Caro,2 Sophie Minjolle,3 Isabelle Jusselin,3 Bruno Pozzetto,1 Radu Crainic,2 and Ronald Colimon3*

Laboratoire de Bactériologie-Virologie, GIMAP, Faculté de Médecine, St-Etienne,1 Laboratoire d'Epidémiologie moléculaire des Entérovirus, Institut Pasteur, Paris,2 Laboratoire de Bactériologie-Virologie, Faculté de Médecine, Rennes, France3

Received 6 June 2002/ Returned for modification 25 September 2002/ Accepted 20 December 2002

We describe a new PCR test (Penter RT-PCR) that recognizes all 64 prototypes of enterovirus. Sixty clinical samples were analyzed in parallel with this Penter RT-PCR and previously described PCR tests: 34 and 32 samples tested positive, respectively. This assay is suitable for use in clinical diagnosis, and its ability to amplify all known serotypes makes it more useful than other consensus PCR tests.

* Corresponding author. Mailing address: Laboratoire de Bactériologie-Virologie, Faculté de Médecine, Université de Rennes 1, 2 avenue du Pr. Léon Bernard, CS 34317, 35043 Rennes cedex, France. Phone: (33) 2 99 28 42 76. Fax: (33) 2 99 28 41 59. E-mail: ronald.colimon{at}univ-rennes1.fr.

Journal of Clinical Microbiology, April 2003, p. 1750-1752, Vol. 41, No. 4
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.4.1750-1752.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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Antimicrob. Agents Chemother. Clin. Microbiol. Rev.
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Copyright © 2003 by the American Society for Microbiology. All rights reserved.