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Journal of Clinical Microbiology, April 2003, p. 1753-1755, Vol. 41, No. 4
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.4.1753-1755.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Molecular Detection of Histoplasma capsulatum var. capsulatum in Human Clinical Samples

Andrea Bracca,1 María Elena Tosello,2 Javier E. Girardini,1 Susana L. Amigot,2,3 Carlos Gomez,2,3 and Esteban Serra1*

Instituto de Biología Molecular y Celular de Rosario, IBR-CONICET, Facultad de Ciencias Bioquímicas y Farmacéuticas,1 Centro de Referencia de Micología, CEREMIC,2 Dirección de Laboratorios, Municipalidad de Rosario, 2000 Rosario, Argentina3

Received 3 June 2002/ Returned for modification 20 August 2002/ Accepted 23 December 2002

We developed a seminested PCR for the diagnosis of histoplasmosis that amplifies a portion of the Histoplasma capsulatum H antigen gene. This assay is highly sensitive and specific, being able to detect genomic material corresponding to less than 10 yeast cells without cross-reaction against other bacterial or fungal pathogens.

* Corresponding author. Mailing address: Instituto de Biología Molecular y Celular de Rosario, IBR-CONICET, Facultad de Ciencias Bioquímicas y Farmacéuticas, Suipacha 531, 2000 Rosario, Argentina. Phone: 54 341 4350661. Fax: 54 341 4390465. E-mail: eserra{at}arnet.com.ar.

Journal of Clinical Microbiology, April 2003, p. 1753-1755, Vol. 41, No. 4
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.4.1753-1755.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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