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Journal of Clinical Microbiology, April 2003, p. 1766-1768, Vol. 41, No. 4
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.4.1766-1768.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Evaluation of a Fluorescence-Based PCR Method for Identification of Serogroup A Meningococci

M. A. Diggle,1 K. Smith,1 E. K. Girvan,2 and S. C. Clarke1,3*

Scottish Meningococcus and Pneumococcus Reference Laboratory, Stobhill Hospital,1 Faculty of Biomedical and Life Sciences, University of Glasgow, Glasgow,3 Department of Microbiology, Wishaw General Hospital, Wishaw, United Kingdom2

Received 21 August 2002/ Returned for modification 2 October 2002/ Accepted 29 December 2002

Standard and fluorescence-based PCR assays were developed for the identification of serogroup A meningococci by detection of the mynA gene. This assay was evaluated using bacterial cultures but provides the sensitivity required for the detection of the mynA gene from bodily fluids during meningococcal disease.

* Corresponding author. Mailing address: Scottish Meningococcus and Pneumococcus Reference Laboratory, North Glasgow University Hospital NHS Trust, Department of Microbiology, Stobhill Hospital, Balornock Rd., Glasgow G21 3UW, United Kingdom. Phone: 44 141 201 3836. Fax: 44 141 201 3663. E-mail: stuart.clarke{at}northglasgow.scot.nhs.uk.

Journal of Clinical Microbiology, April 2003, p. 1766-1768, Vol. 41, No. 4
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.4.1766-1768.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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