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Journal of Clinical Microbiology, April 2003, p. 1801-1804, Vol. 41, No. 4
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.4.1801-1804.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

New Method for Typing Staphylococcus aureus Strains: Multiple-Locus Variable-Number Tandem Repeat Analysis of Polymorphism and Genetic Relationships of Clinical Isolates

Artur Sabat,1 Jolanta Krzyszton-Russjan,2 Wojciech Strzalka,1 Renata Filipek,1 Klaudia Kosowska,1 Waleria Hryniewicz,2 James Travis,3 and Jan Potempa1,3*

Department of Microbiology, Institute of Molecular Biology and Biotechnology, Jagiellonian University, Cracow,1 Sera and Vaccines Central Research Laboratory, Warsaw, Poland,2 Department of Biochemistry and Molecular Biology, University of Georgia, Athens, Georgia 306023

Received 15 July 2002/ Returned for modification 7 August 2002/ Accepted 7 January 2003

The PCR-based methodology applied to multiple-locus variable numbers of tandem repeat (VNTR) analysis was recently shown to be a useful technique for the molecular typing of clinical isolates of several bacterial species. We have adopted this method for the molecular typing of methicillin-resistant Staphylococcus aureus. Five staphylococcal VNTR loci (sdr, clfA, clfB, ssp, and spa) were subjected to analysis, and it was shown that the method allows typing of S. aureus strains with the discriminatory power and reproducibility of pulsed-field gel electrophoresis while at the same time being rapid and applicable to analysis of large numbers of isolates.

* Corresponding author. Mailing address: Department of Biochemistry and Molecular Biology, University of Georgia, Life Science Bldg., East Green St., Athens, GA 30602. Phone: (706) 542-1713. Fax: (706) 542-3719. E-mail: potempa{at}arches.uga.edu.

Journal of Clinical Microbiology, April 2003, p. 1801-1804, Vol. 41, No. 4
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.4.1801-1804.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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