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Journal of Clinical Microbiology, May 2003, p. 1888-1893, Vol. 41, No. 5
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.5.1888-1893.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Multicenter Evaluation of Use of Dried Blood and Plasma Spot Specimens in Quantitative Assays for Human Immunodeficiency Virus RNA: Measurement, Precision, and RNA Stability

Don Brambilla,1 Cheryl Jennings,2 Grace Aldrovandi,3 James Bremer,2 Anne Marie Comeau,4 Sharon A. Cassol,5 Ruth Dickover,6 J. Brooks Jackson,7 Jane Pitt,8 John L. Sullivan,9 Ann Butcher,10 Lynell Grosso,11 Patricia Reichelderfer,12 and Susan A. Fiscus13*

New England Research Center, Waltham, Massachusetts,1 Rush-Presbyterian Hospital, Chicago, Illinois,2 University of Alabama, Birmingham, Alabama,3 New England Newborn Screening Program, University of Massachusetts, Boston, Massachusetts,4 Africa Centre, Durban, South Africa,5 University of California, Los Angeles, California,6 Johns Hopkins University, Baltimore, Maryland,7 Columbia University, New York, New York,8 University of Massachusetts, Worcester, Massachusetts,9 Roche Molecular Systems, Pleasanton, California,10 bioMerieux, Durham, North Carolina,11 National Institute of Child Health and Development, Bethesda, Maryland,12 University of North Carolina, Chapel Hill, North Carolina,13

Received 3 July 2002/ Returned for modification 20 September 2002/ Accepted 29 January 2003

Eleven laboratories evaluated the use of dried blood and plasma spots for quantitation of human immunodeficiency virus (HIV) RNA by two commercially available RNA assays, the Roche Amplicor HIV-1 Monitor and the bioMerieux NucliSens HIV-1 QT assays. The recovery of HIV RNA was linear over a dynamic range extending from 4,000 to 500,000 HIV type 1 RNA copies/ml. The Monitor assay appeared to have a broader dynamic range and seemed more sensitive at lower concentrations. However, the NucliSens assay gave more consistent results and could be performed without modification of the kit. HIV RNA was stable in dried whole blood or plasma stored at room temperature or at -70°C for up to 1 year. Dried blood and dried plasma spots can be used as an easy and inexpensive means for the collection and storage of specimens under field conditions for the diagnosis of HIV infection and the monitoring of antiretroviral therapy.

* Corresponding author. Mailing address: Department of Microbiology and Immunology, University of North Carolina, 709 Mary Ellen Jones Building, Chapel Hill, NC 28599-7140. Phone: (919) 966-6872. Fax: (919) 966-9873. E-mail: fiscussa{at}med.unc.edu.

Journal of Clinical Microbiology, May 2003, p. 1888-1893, Vol. 41, No. 5
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.5.1888-1893.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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