This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Loens, K. Articles by Goossens, H. Search for Related Content PubMed PubMed Citation Articles by Loens, K. Articles by Goossens, H.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, May 2003, p. 1971-1976, Vol. 41, No. 5
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.5.1971-1976.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Improved Detection of Rhinoviruses by Nucleic Acid Sequence-Based Amplification after Nucleotide Sequence Determination of the 5' Noncoding Regions of Additional Rhinovirus Strains

Department of Microbiology, University of Antwerp UIA, Antwerp, Belgium,¹ Organon Teknika BV, Boxtel, The Netherlands²

Received 11 February 2002/ Returned for modification 4 August 2002/ Accepted 19 February 2003

The isothermal nucleic acid sequence-based amplification (NASBA) system was applied for the detection of rhinoviruses using primers targeted at the 5' noncoding region (5' NCR) of the viral genome. The nucleotide sequence of the 5' NCRs of 34 rhinovirus isolates was determined to map the most conserved regions and design more appropriate primers and probes. The assay amplified RNA extracted from 30 rhinovirus reference strains and 88 rhinovirus isolates, it did not amplify RNA from 49 enterovirus isolates and other respiratory viruses. The assay allows one to discriminate between group A and B rhinoviruses. Sensitivities for the detection of group B and group A rhinoviruses was 20 and 200 50% tissue culture infective doses, respectively.

This article has been cited by other articles:

• Loens, K., Goossens, H., de Laat, C., Foolen, H., Oudshoorn, P., Pattyn, S., Sillekens, P., Ieven, M. (2006). Detection of Rhinoviruses by Tissue Culture and Two Independent Amplification Techniques, Nucleic Acid Sequence-Based Amplification and Reverse Transcription-PCR, in Children with Acute Respiratory Infections during a Winter Season. J. Clin. Microbiol. 44: 166-171 [Abstract] [Full Text]  
• Deffernez, C., Wunderli, W., Thomas, Y., Yerly, S., Perrin, L., Kaiser, L. (2004). Amplicon Sequencing and Improved Detection of Human Rhinovirus in Respiratory Samples. J. Clin. Microbiol. 42: 3212-3218 [Abstract] [Full Text]