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Journal of Clinical Microbiology, May 2003, p. 2027-2032, Vol. 41, No. 5
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.5.2027-2032.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Two International Methicillin-Resistant Staphylococcus aureus Clones Endemic in a University Hospital in Patras, Greece

M. Aires de Sousa,1 C. Bartzavali,2 I. Spiliopoulou,1,2 I. Santos Sanches,1,3 M. I. Crisóstomo,1,4 and H. de Lencastre1,4*

Laboratório de Genética Molecular, Instituto de Tecnologia Química e Biológica da Universidade Nova de Lisboa, Oeiras,1 Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa (FCT/UNL), Monte da Caparica, Portugal,3 Department of Microbiology, School of Medicine, University of Patras, Patras, Greece,2 Laboratory of Microbiology, The Rockefeller University, New York, New York4

Received 6 December 2002/ Returned for modification 20 January 2003/ Accepted 14 February 2003

Pulsed-field gel electrophoresis (PFGE) of SmaI macrofragments and hybridization of ClaI digests with the mecA- and Tn554-specific DNA probes were used to define the endemic clones of methicillin-resistant Staphylococcus aureus (MRSA) among strains collected in 1993 and 1998 to 2000 at the University Hospital of Patras, Patras, Greece. Representatives of each clonal type were analyzed by spaA typing, multilocus sequence typing (MLST), and staphylococcal chromosomal cassette mec (SCCmec) typing. The results indicated the existence of two successive international MRSA clones: (i) a clonal type with PFGE type A, sequence type (ST) 30 (ST30), and SCCmec type IV, which was very similar to a clone widely spread in the United Kingdom, Mexico, and Finland, and (ii) a clonal type with PFGE type B, ST239, and SCCmec III, which was related to the Brazilian clone. Both clones seem to be widespread in Greece as well. A novel MRSA clone is also described and is characterized by a new MLST type (ST80) associated with SCCmec type IV and with the presence of Panton-Valentine leukocidin genes.

* Corresponding author. Mailing address: The Rockefeller University, 1230 York Ave., New York, NY 10021. Phone: (212) 327-8278. Fax: (212) 327-8688. E-mail: lencash{at}mail.rockefeller.edu.

Journal of Clinical Microbiology, May 2003, p. 2027-2032, Vol. 41, No. 5
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.5.2027-2032.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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