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Journal of Clinical Microbiology, May 2003, p. 2132-2134, Vol. 41, No. 5
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.5.2132-2134.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Comparison of Lateral-Flow Immunoassay and Enzyme Immunoassay with Viral Culture for Rapid Detection of Influenza Virus in Nasal Wash Specimens from Children

Andreea C. Cazacu,1 Jewel Greer,1,2 Moe Taherivand,1,2 and Gail J. Demmler1,2*

Department of Pediatrics, Baylor College of Medicine,1 Diagnostic Virology Laboratory, Texas Children's Hospital, Houston, Texas 770302

Received 13 September 2002/ Returned for modification 29 November 2002/ Accepted 29 January 2003

The performance of two commercially available rapid test kits for influenza virus detection was compared to that of viral culture by using 356 nasal wash specimens collected during the 2001 to 2002 influenza season. Overall, the two rapid tests were easy to perform and showed comparable sensitivities (70.4 and 72.2%) and specificities (97.7 and 98.3%); for both test kit groups, most of the specimens that yielded false-negative results were found to be growing influenza B virus.

* Corresponding author. Mailing address: Pediatric Infectious Diseases, Feigin Center MC3-2371, Texas Children's Hospital, 6621 Fannin, Houston, TX 77030. Phone: (832) 824-4330. Fax: (832) 825-4347. E-mail: gdemmler{at}bcm.tmc.edu.

Journal of Clinical Microbiology, May 2003, p. 2132-2134, Vol. 41, No. 5
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.5.2132-2134.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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