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Journal of Clinical Microbiology, May 2003, p. 2138-2140, Vol. 41, No. 5
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.5.2138-2140.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Multiplex PCR for Detection of Three Plasmid-Borne Genes of Enteroaggregative Escherichia coli Strains

Jorge F. Cerna,1 James P. Nataro,2 and Teresa Estrada-Garcia1*

Department of Molecular Biomedicine, CINVESTAV-IPN, Zacatenco, Mexico DF 07360, Mexico,1 Center for Vaccine Development, Department of Pediatrics and Medicine, University of Maryland, School of Medicine, Baltimore, Maryland2

Received 9 December 2002/ Returned for modification 29 January 2003/ Accepted 13 February 2003

We developed a novel multiplex PCR assay for enteroaggregative Escherichia coli (EAEC) detection, by using three plasmid-borne genes (the aggregative adherence [AA] probe, aap, and aggR). One or more of the loci were detected in 24 (86%) of 28 patient isolates analyzed. The multiplex PCR assay is a fast, convenient, and sensitive molecular test to detect EAEC.

* Corresponding author. Mailing address: Department of Molecular Biomedicine, CINVESTAV-IPN, Av. Instituto Politecnico Nacional 2508, Zacatenco, Mexico DF 07360, Mexico. Phone: (5255) 5-747-3327. Fax: (5255) 5-747-7134. E-mail: testrada{at}mail.cinvestav.mx.

Journal of Clinical Microbiology, May 2003, p. 2138-2140, Vol. 41, No. 5
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.5.2138-2140.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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