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Journal of Clinical Microbiology, May 2003, p. 2174-2175, Vol. 41, No. 5
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.5.2174-2175.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Pilot Study of COBAS PCR and Ligase Chain Reaction for Detection of Rectal Infections Due to Chlamydia trachomatis

Matthew R. Golden,1,2* Sabina G. Astete,1 Rosa Galvan,3 Aldo Lucchetti,3 Jorge Sanchez,3 Connie L. Celum,1 William L. H. Whittington,1 Walter E. Stamm,1 King K. Holmes,1 and Patricia A. Totten1

Division of Infectious Diseases and the Center for AIDS & STD, University of Washington, Seattle,1 Public Health—Seattle and King County STD Program, Seattle, Washington,2 Asociación Civil Impacta Salud y Educación, Lima, Peru3

Received 2 December 2002/ Returned for modification 12 February 2003/ Accepted 18 February 2003

We tested rectal specimens from men who have sex with men for Chlamydia trachomatis by using COBAS PCR (Roche Diagnostics) and ligase chain reaction LCR (Abbott laboratories) and compared three PCR specimen-processing procedures. Chlamydiae were detected by one or more procedures in 22 of 186 specimens. All three PCR tests were positive for 17 specimens, all of which also tested positive by LCR.

* Corresponding author. Mailing address: Harborview Medical Center Box 359777, 325 9th Ave., Seattle, WA 98104. Phone: (206) 731-6829. Fax: (206) 731-4151. E-mail: golden{at}u.washington.edu.

Journal of Clinical Microbiology, May 2003, p. 2174-2175, Vol. 41, No. 5
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.5.2174-2175.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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