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Journal of Clinical Microbiology, May 2003, p. 2191-2196, Vol. 41, No. 5
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.5.2191-2196.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Multilocus Sequence Typing Has Better Discriminatory Ability for Typing Vibrio cholerae than Does Pulsed-Field Gel Electrophoresis and Provides a Measure of Phylogenetic Relatedness

Mamuka Kotetishvili,1,2 O. Colin Stine,1 Yuansha Chen,1 Arnold Kreger,1 Alexander Sulakvelidze,1 Shanmuga Sozhamannan,1,{dagger} and J. Glenn Morris, Jr.1*

Department of Epidemiology and Preventive Medicine, University of Maryland School of Medicine, Baltimore, Maryland 21201,1 Kanchaveli Institute of Plant Protection, Tbilisi, Georgia 3800772

Received 20 November 2002/ Returned for modification 10 January 2003/ Accepted 10 February 2003

Twenty-two Vibrio cholerae isolates, including some from "epidemic" (O1 and O139) and "nonepidemic" serogroups, were characterized by pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) by using three housekeeping genes, gyrB, pgm, and recA; sequence data were also obtained for the virulence-associated genes tcpA, ctxA, and ctxB. Even with the small number of loci used, MLST had better discriminatory ability than did PFGE. On MLST analysis, there was clear clustering of epidemic serogroups; much greater diversity was seen among tcpA- and ctxAB-positive V. cholerae strains from other, nonepidemic serogroups, with a number of tcpA and ctxAB alleles identified.

* Corresponding author. Mailing address: Department of Epidemiology and Preventive Medicine, University of Maryland School of Medicine, 10 South Pine St., Baltimore, Maryland 21201. Phone: (410) 706-4580. Fax: (410) 706-4581. E-mail: jmorris{at}epi.umaryland.edu.

{dagger} Present address: Intralytix, Inc., Baltimore, MD 21202.

Journal of Clinical Microbiology, May 2003, p. 2191-2196, Vol. 41, No. 5
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.5.2191-2196.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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