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Journal of Clinical Microbiology, May 2003, p. 2209-2212, Vol. 41, No. 5
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.5.2209-2212.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Mutations in the rpoB Gene of Multidrug-Resistant Mycobacterium tuberculosis Isolates from China

Jun Yue,1,2 Wei Shi,1,3 Jingping Xie,1 Yao Li,1,3 Erliang Zeng,1,3 and Honghai Wang1*

State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Science, Fudan University,1 Shanghai Pneumology Hospital, Shanghai 200433,2 Shanghai Institute of Biostar Genechip, Shanghai 200092, People's Republic of China3

Received 11 September 2002/ Returned for modification 18 November 2002/ Accepted 4 February 2003

Mutations in the 81-bp rifampin resistance determining region (RRDR) and mutation V176F locating at the beginning of the ropB gene were analyzed by DNA sequencing of 86 Mycobacterium tuberculosis clinical isolates (72 resistant and 14 sensitive) from different parts of China. Sixty-five mutations of 22 distinct kinds, 21 point mutations, and 1 insertion were found in 65 of 72 resistant isolates. The most common mutations were in codons 531 (41%), 526 (40%), and 516 (4%). Mutations were not found in seven (10%) of the resistant isolates. Six new alleles within the RRDR, along with five novel mutations outside the RRDR, are reported. None of isolates contained the V176 mutation.

* Corresponding author. Mailing address: State Key Laboratory of Genetic Engineering Institute of Genetics, Fudan University, 220 Handan Rd., Shanghai 200433, People's Republic of China. Phone: (8621) 65643777. Fax: (8621) 65648376. E-mail: hhwang{at}fudan.edu.

Journal of Clinical Microbiology, May 2003, p. 2209-2212, Vol. 41, No. 5
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.5.2209-2212.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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