This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Deng, Y.
Right arrow Articles by Clarke, I. N.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Deng, Y.
Right arrow Articles by Clarke, I. N.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, June 2003, p. 2300-2305, Vol. 41, No. 6
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.6.2300-2305.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Studies of Epidemiology and Seroprevalence of Bovine Noroviruses in Germany

Y. Deng,1 C. A. Batten,1 B. L. Liu,1 P. R. Lambden,1 M. Elschner,2 H. Günther,2 P. Otto,2 P. Schnürch,3 W. Eichhorn,4 W. Herbst,5 and I. N. Clarke1*

Molecular Microbiology Group, University Medical School, Southampton General Hospital, Southampton SO16 6YD, United Kingdom,1 Federal Research Centre for Virus Diseases of Animals, 07743 Jena,2 Animal Health Service of Thuringia, 99947 Bad Langensalza,3 Institute for Medical Microbiology and Infectious Diseases, Faculty of Veterinary Medicine, Ludwig-Maximillian University, 80538 Munich,4 Institute of Hygiene and Infectious Diseases of Animals, Department of Veterinary Medicine, Justus-Liebig University, 35392 Giessen, Germany5

Received 27 December 2002/ Returned for modification 29 January 2003/ Accepted 5 March 2003

Jena virus (JV) is a bovine enteric calicivirus that causes diarrhea in calves. The virus is approximately 30 nm in diameter and has a surface morphology similar to the human Norwalk virus. The genome sequence of JV was recently described, and the virus has been assigned to the genus Norovirus of the family Caliciviridae. In the present study, the JV capsid gene encoded by open reading frame 2 was cloned into the baculovirus transfer vector pFastBac 1, and this was used to transform Escherichia coli to generate a recombinant bacmid. Transfection of insect cells with the recombinant baculovirus DNA resulted in expression of the JV capsid protein. The recombinant JV capsid protein undergoes self-assembly into virus-like particles (VLPs) similar to JV virions in size and appearance. JV VLPs were released into the cell culture supernatant, concentrated, and then purified by CsCl equilibrium gradient centrifugation. Purified JV VLPs were used to hyperimmunize laboratory animals. An antigen capture enzyme-linked immunosorbent assay (ELISA) was developed and characterized initially with clinical specimens containing defined human noroviruses and bovine diarrheal samples from calves experimentally infected with JV; the ELISA was specific only for JV. The ELISA was used to screen 381 diarrheal samples collected from dairy herds in Thuringia, Hesse, and Bavaria, Germany, from 1999 to 2002; 34 of these samples (8.9%) were positive for JV infection. The unexpectedly high prevalence of JV was confirmed in a seroepidemiological study using 824 serum or plasma samples screened using an anti-JV ELISA, which showed that 99.1% of cattle from Thuringia have antibodies to JV.

* Corresponding author. Mailing address: Molecular Microbiology Group, University Medical School, Southampton General Hospital, Southampton SO16 6YD, United Kingdom. Phone: 44 023 80796975. Fax: 44 023 80796992. E-mail: inc{at}soton.ac.uk.

Journal of Clinical Microbiology, June 2003, p. 2300-2305, Vol. 41, No. 6
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.6.2300-2305.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Almanza, H., Cubillos, C., Angulo, I., Mateos, F., Caston, J. R., van der Poel, W. H. M., Vinje, J., Barcena, J., Mena, I. (2008). Self-Assembly of the Recombinant Capsid Protein of a Swine Norovirus into Virus-Like Particles and Evaluation of Monoclonal Antibodies Cross-Reactive with a Human Strain from Genogroup II. J. Clin. Microbiol. 46: 3971-3979 [Abstract] [Full Text]  
  • Oliver, S. L., Wood, E., Asobayire, E., Wathes, D. C., Brickell, J. S., Elschner, M., Otto, P., Lambden, P. R., Clarke, I. N., Bridger, J. C. (2007). Serotype 1 and 2 Bovine Noroviruses Are Endemic in Cattle in the United Kingdom and Germany. J. Clin. Microbiol. 45: 3050-3052 [Abstract] [Full Text]  
  • Wolf, S., Williamson, W. M., Hewitt, J., Rivera-Aban, M., Lin, S., Ball, A., Scholes, P., Greening, G. E. (2007). Sensitive Multiplex Real-Time Reverse Transcription-PCR Assay for the Detection of Human and Animal Noroviruses in Clinical and Environmental Samples. Appl. Environ. Microbiol. 73: 5464-5470 [Abstract] [Full Text]  
  • Farkas, T., Deng, X., Ruiz-Palacios, G., Morrow, A., Jiang, X. (2006). Development of an enzyme immunoassay for detection of sapovirus-specific antibodies and its application in a study of seroprevalence in children.. J. Clin. Microbiol. 44: 3674-3679 [Abstract] [Full Text]  
  • Oliver, S. L., Batten, C. A., Deng, Y., Elschner, M., Otto, P., Charpilienne, A., Clarke, I. N., Bridger, J. C., Lambden, P. R. (2006). Genotype 1 and genotype 2 bovine noroviruses are antigenically distinct but share a cross-reactive epitope with human noroviruses.. J. Clin. Microbiol. 44: 992-998 [Abstract] [Full Text]  
  • Farkas, T., Nakajima, S., Sugieda, M., Deng, X., Zhong, W., Jiang, X. (2005). Seroprevalence of Noroviruses in Swine. J. Clin. Microbiol. 43: 657-661 [Abstract] [Full Text]  
  • Han, M. G., Wang, Q., Smiley, J. R., Chang, K. O., Saif, L. J. (2005). Self-Assembly of the Recombinant Capsid Protein of a Bovine Norovirus (BoNV) into Virus-Like Particles and Evaluation of Cross-Reactivity of BoNV with Human Noroviruses. J. Clin. Microbiol. 43: 778-785 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»