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Journal of Clinical Microbiology, June 2003, p. 2367-2371, Vol. 41, No. 6
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.6.2367-2371.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Precise Characterization of Norovirus (Norwalk-Like Virus)-Specific Monoclonal Antibodies with Broad Reactivity

Tomoko Yoda,1* Yasuhiko Suzuki,2 Yoshitake Terano,3 Kenji Yamazaki,4 Naomi Sakon,4 Tsuyoshi Kuzuguchi,5 Hajime Oda,6 and Teizo Tsukamoto1

Division of Food Microbiology,1 Division of Pathology,2 Division of Virology,4 Division of Industrial Health, Osaka Prefectural Institute of Public Health,6 Department of Pharmacology, Osaka University of Pharmaceutical Sciences, Osaka,3 Department of Molecular Microbiology, Gifu Prefectural Institute for Bio-Industrial Technology, Gifu, Japan5

Received 8 July 2002/ Returned for modification 29 November 2002/ Accepted 3 March 2003

We have been characterizing monoclonal antibodies against Norovirus (Norwalk-like virus). In the course of our study, two monoclonal antibodies generated against Norovirus genogroup II capsid protein were found to react not only to genogroup II but also to genogroup I recombinant capsid proteins. In addition, we showed that these two monoclonal antibodies reacted to a 40-amino-acid-fragment located close to the N-terminal region of genogroup II Norovirus. Similar reactivity was observed with the equivalent region of genogroup I Norovirus. In this study, we confirmed that the epitopes of the two monoclonal antibodies existed within an 11-amino-acid peptide. To obtain an idea of the reactive ranges of the two monoclonal antibodies toward different strains of Norovirus, their reactivities were investigated using 16 types of peptide constructed according to the data in GenBank and 8 recombinant capsid proteins (7 whole capsid proteins and 1 short [80-amino-acid] protein fragment). A characteristic broad reactivity of the two monoclonal antibodies is clearly shown by the results of this study. Thus, these monoclonal antibodies could be useful tools for detecting a broad range of Norovirus strains.

* Corresponding author. Present address: Division of Bacteriology, Osaka Prefectural Institute of Public Health, Nakamichi, Higashinari-ku, Osaka City 537-0025, Japan. Phone: 81-6-6972-1321, ext. 361. Fax: 81-6-6972-2393. E-mail: yoda{at}iph.pref.osaka.jp. {dagger} Present address: Department of Microbiology and Pathology, Faculty of Medicine, Tottori University, Yonago City, Japan.

Journal of Clinical Microbiology, June 2003, p. 2367-2371, Vol. 41, No. 6
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.6.2367-2371.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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