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Journal of Clinical Microbiology, June 2003, p. 2385-2390, Vol. 41, No. 6
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.6.2385-2390.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Survey of emm Gene Sequences from Pharyngeal Streptococcus pyogenes Isolates Collected in Spain and Their Relationship with Erythromycin Susceptibility

Sebastián Albertí,1* César García-Rey,2 María A. Domínguez,3 Lorenzo Aguilar,2 Emilia Cercenado,4 Miguel Gobernado,5 Adela García-Perea,6 and Spanish Surveillance Group for Respiratory Pathogens {dagger}

Unidad de Investigación, Hospital Universitario Son Dureta, Palma de Mallorca,1 Departamento Médico, GlaxoSmithKline, Tres Cantos,2 Servicio de Microbiología, Hospital de Bellvitge, Barcelona,3 Servicio de Microbiología, Hospital General Universitario Gregorio Marañón,4 Servicio de Microbiología, Hospital Universitario La Paz, Madrid,6 Servicio de Microbiología, Hospital Universitario La Fe, Valencia, Spain5

Received 15 November 2002/ Returned for modification 19 January 2003/ Accepted 19 March 2003

We conducted a nationwide survey of the variable 5' emm (M protein gene) sequences from 614 pharyngeal Streptococcus pyogenes isolates susceptible (299 isolates) and resistant (315 isolates) to erythromycin that were isolated in Spain from 1996 to 1999. Almost 98% of these isolates had emm sequences in agreement with previously recorded M antigen association. We only identified a new 5' emm sequence in 17 isolates. Nine different emm types accounted for 85% of the S. pyogenes isolates susceptible to erythromycin. By contrast, only 3 emm types accounted for 70% of the erythromycin-resistant isolates. Further characterization of these isolates by ribotyping and pulsed-field gel electrophoresis indicated that high frequency of erythromycin resistance in Spain is due to few clones.

* Corresponding author. Mailing address: Unidad de Investigación, Hospital Son Dureta, Andrea Doria, 55, Palma de Mallorca 07014, Spain. Phone: 34-971-175334. Fax: 34-971-175228. E-mail: salberti{at}hsd.es.

{dagger} Participants of the Spanish Surveillance Group for Respiratory Pathogens are not listed, but the list is available from the corresponding author.

Journal of Clinical Microbiology, June 2003, p. 2385-2390, Vol. 41, No. 6
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.6.2385-2390.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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