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Journal of Clinical Microbiology, June 2003, p. 2454-2457, Vol. 41, No. 6
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.6.2454-2457.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Evaluation of Repetitive Element Sequence-Based PCR as a Molecular Typing Method for Clostridium difficile

Patrizia Spigaglia and Paola Mastrantonio*

Bacteriology and Medical Mycology Laboratory, Istituto Superiore di Sanità, Rome, Italy

Received 18 November 2002/ Returned for modification 4 February 2003/ Accepted 10 March 2003

Repetitive element sequence-based PCR (rep-PCR) is a typing method that enables the generation of DNA fingerprinting that discriminates bacterial strains. In this study, we evaluated the applicability of rep-PCR in typing Clostridium difficile clinical isolates. The results obtained by rep-PCR were compared with those obtained by pulsed-field gel electrophoresis (PFGE) and PCR ribotyping. A high correspondence between pattern differentiations produced by rep-PCR and PFGE was observed, whereas PCR ribotyping showed a lower level of discriminatory power.

* Corresponding author. Mailing address: Laboratorio di Batteriologia e Micologia Medica, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161 Rome, Italy. Phone: 39-06-49902335. Fax: 39-06-49387112. E-mail: pmastran{at}iss.it.

Journal of Clinical Microbiology, June 2003, p. 2454-2457, Vol. 41, No. 6
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.6.2454-2457.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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