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Journal of Clinical Microbiology, June 2003, p. 2471-2476, Vol. 41, No. 6
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.6.2471-2476.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Identification by Subtractive Hybridization of a Novel Insertion Element Specific for Two Widespread Burkholderia cepacia Genomovar III Strains

Lixia Liu, Theodore Spilker, Tom Coenye,{dagger} and John J. LiPuma*

Department of Pediatrics and Communicable Diseases, University of Michigan Medical School, Ann Arbor, Michigan 48109-0646

Received 9 December 2002/ Returned for modification 1 February 2003/ Accepted 25 February 2003

Species of the Burkholderia cepacia complex cause chronic and life-threatening infections in persons with cystic fibrosis. Epidemic strains infect multiple patients, reside primarily in genomovar III, and have an apparent enhanced capacity for human infection and/or interpatient transmission. By using subtractive hybridization, a novel insertion element, designated IS1363, was identified in epidemic strain PHDC, known to infect many cystic fibrosis patients in the mid-Atlantic region of the United States. IS1363 was also found in most isolates of the ET12 lineage, responsible for infecting large numbers of patients in Ontario, Canada, and the United Kingdom. Southern blot analysis demonstrated that whereas multiple copies of IS1363 were present in strain PHDC, only one copy was present in ET12 isolates. IS1363 was used to probe a collection of 943 B. cepacia complex isolates, representing all nine genomovars, recovered from 761 cystic fibrosis patients or the natural environment. IS1363 was not found in other genomovar III strains and, with the exception of B. ambifaria, was absent from other B. cepacia complex species. Genotyping analyses of all IS1363-positive isolates demonstrated that strain PHDC was more widely distributed in the United States than previously appreciated; 212 cystic fibrosis patients in 24 states were identified as being infected with PHDC.

* Corresponding author. Mailing address: University of Michigan Medical School, 8323 MSRB III, Box 0646, 1150 W. Medical Center Dr., Ann Arbor, MI 48109-0646. Phone: (734) 936-9767. Fax: (734) 764-4279. E-mail: jlipuma{at}umich.edu.

{dagger} Present address: Laboratorium voor Microbiologie, Universiteit Gent, B-9000 Ghent, Belgium.

Journal of Clinical Microbiology, June 2003, p. 2471-2476, Vol. 41, No. 6
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.6.2471-2476.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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