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Journal of Clinical Microbiology, June 2003, p. 2477-2482, Vol. 41, No. 6
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.6.2477-2482.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Dissemination of SHV-12 and Characterization of New AmpC-Type Beta-Lactamase Genes among Clinical Isolates of Enterobacter Species in Korea

Sang Hee Lee,1* Jae Young Kim,2 Sang Heum Shin,1 Young Jun An,1 Young Wook Choi,1 Yeun Chang Jung,1 Ha Il Jung,1 Eui Suk Sohn,1 Seok Hoon Jeong,3,4 and Kye Joon Lee2

Department of Genetic Engineering, Youngdong University, Chungbuk 370-701,1 Department of Clinical Pathology, Kosin University College of Medicine, Pusan,3 Research Institute of Bacterial Resistance, Yonsei University College of Medicine,4 Department of Microbiology and Biotechnology, Graduate School of Biological Sciences, Seoul National University, Seoul, South Korea2

Received 17 September 2002/ Returned for modification 5 November 2002/ Accepted 27 February 2003

To determine the prevalence and genotype of an extended-spectrum beta-lactamase and new chromosomal AmpC beta-lactamases among clinical isolates of Enterobacter species, we performed antibiotic susceptibility testing, pI determination, induction tests, transconjugation, enterobacterial repetitive consensus (ERIC) PCR, sequencing, and phylogenetic analysis. Among the 51 clinical isolates collected from a university hospital in Korea, 6 isolates have been shown to produce SHV-12 and inducible AmpC beta-lactamases. These also included three isolates producing TEM-1b and one strain carrying TEM-1b and CMY-type beta-lactamases with a pI of 8.0. The results from ERIC PCR revealed that six isolates were genetically unrelated, suggesting that dissemination of SHV-12 was responsible for the spread of resistance to extended-spectrum beta-lactams in Korea. Six genes of inducible AmpC beta-lactamases that are responsible for the resistance to cephamycins (cefoxitin and cefotetan), amoxicillin, cephalothin, and amoxicillin-clavulanic acid were cloned and characterized. A 1,165-bp DNA fragment containing the ampC genes was sequenced and found to have an open reading frame coding for a 381-amino-acid beta-lactamase. The nucleotide sequence of four ampC genes (blaEcloK992004.1, blaEcloK995120.1, blaEcloK99230, and blaEareK9911729) shared considerable homology with that of AmpC-type class C beta-lactamase genes of gram-negative bacteria, especially that of the chromosomal ampC gene (blaEcloMHN1) of Enterobacter cloacae MHN1 (99.9, 99.7, 99.6, and 99.6% identity, respectively). The sequences of two ampC genes (blaEcloK9973 and blaEcloK9914325) showed close similarity to the chromosomal ampC gene (blaEcloQ908R) of E. cloacae Q908R (99.7% identity). The results from phylogenetic analysis suggested that six ampC genes could originate from blaEcloMHN1 or blaEcloQ908R.

* Corresponding author. Mailing address: Department of Genetic Engineering, Youngdong University, Chungbuk 370-701, South Korea. Phone: 82-43-740-1112. Fax: 82-43-740-1109. E-mail: sanghee{at}youngdong.ac.kr.

Journal of Clinical Microbiology, June 2003, p. 2477-2482, Vol. 41, No. 6
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.6.2477-2482.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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Antimicrob. Agents Chemother. Clin. Microbiol. Rev.
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Copyright © 2003 by the American Society for Microbiology. All rights reserved.