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Journal of Clinical Microbiology, June 2003, p. 2530-2536, Vol. 41, No. 6
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.6.2530-2536.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Multilocus Sequence Typing System for Group B Streptococcus

Nicola Jones,1* John F. Bohnsack,2 Shinji Takahashi,3 Karen A. Oliver,1 Man-Suen Chan,4 Frank Kunst,5 Philippe Glaser,5 Christophe Rusniok,5 Derrick W. M. Crook,1 Rosalind M. Harding,6 Naiel Bisharat,1 and Brian G. Spratt7

Nuffield Department of Clinical Laboratory Sciences,1 Paediatric Molecular Medicine, Institute for Molecular Medicine, John Radcliffe Hospital, Oxford OX3 9DU,4 The Peter Medawar Building for Pathogen Research, University of Oxford, Oxford OX1 3SY,6 Department of Infectious Disease Epidemiology, Faculty of Medicine, Imperial College, St. Mary's Hospital, London W2 1PG, United Kingdom,7 Department of Pediatrics, University of Utah Health Sciences Center, Salt Lake City, Utah 84132,2 Division of Microbiology, Joshi-Eiyoh University, Chiyoda, Sakado, Saitama 350-0288, Japan,3 Laboratoire de Génomique des Microorganismes Pathogènes, Institut Pasteur, 75724 Paris Cedex 15, France5

Received 29 January 2003/ Returned for modification 7 March 2003/ Accepted 17 March 2003

A multilocus sequence typing (MLST) system was developed for group B streptococcus (GBS). The system was used to characterize a collection (n = 152) of globally and ecologically diverse human strains of GBS that included representatives of capsular serotypes Ia, Ib, II, III, V, VI, and VIII. Fragments (459 to 519 bp) of seven housekeeping genes were amplified by PCR for each strain and sequenced. The combination of alleles at the seven loci provided an allelic profile or sequence type (ST) for each strain. A subset of the strains were characterized by restriction digest patterning, and these results were highly congruent with those obtained with MLST. There were 29 STs, but 66% of isolates were assigned to four major STs. ST-1 and ST-19 were significantly associated with asymptomatic carriage, whereas ST-23 included both carried and invasive strains. All 44 isolates of ST-17 were serotype III clones, and this ST appeared to define a homogeneous clone that was strongly associated with neonatal invasive infections. The finding that isolates with different capsular serotypes had the same ST suggests that recombination occurs at the capsular locus. A web site for GBS MLST was set up and can be accessed at http://sagalactiae.mlst.net. The GBS MLST system offers investigators a valuable typing tool that will promote further investigation of the population biology of this organism.

* Corresponding author. Mailing address: Nuffield Department of Clinical Laboratory Sciences, Level 7, John Radcliffe Hospital, Oxford OX3 9DU, United Kingdom. Phone: 44 1865 220855. Fax: 44 1865 220890. E-mail: nicola.jones{at}ndcls.ox.ac.uk.

Journal of Clinical Microbiology, June 2003, p. 2530-2536, Vol. 41, No. 6
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.6.2530-2536.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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