This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Gorkiewicz, G.
Right arrow Articles by Zechner, E. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gorkiewicz, G.
Right arrow Articles by Zechner, E. L.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, June 2003, p. 2537-2546, Vol. 41, No. 6
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.6.2537-2546.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Species-Specific Identification of Campylobacters by Partial 16S rRNA Gene Sequencing

Gregor Gorkiewicz,1* Gebhard Feierl,2 Caroline Schober,1 Franz Dieber,3 Josef Köfer,3 Rudolf Zechner,1 and Ellen L. Zechner1

Institute of Molecular Biology, Biochemistry and Microbiology,1 Institute of Hygiene, Karl-Franzens University,2 Department of Veterinary Administration in Styria, Graz, Austria3

Received 4 November 2002/ Returned for modification 8 January 2003/ Accepted 4 March 2003

Species-specific identification of campylobacters is problematic, primarily due to the absence of suitable biochemical assays and the existence of atypical strains. 16S rRNA gene (16S rDNA)-based identification of bacteria offers a possible alternative when phenotypic tests fail. Therefore, we evaluated the reliability of 16S rDNA sequencing for the species-specific identification of campylobacters. Sequence analyses were performed by using almost 94% of the complete 16S rRNA genes of 135 phenotypically characterized Campylobacter strains, including all known taxa of this genus. It was shown that 16S rDNA analysis enables specific identification of most Campylobacter species. The exception was a lack of discrimination among the taxa Campylobacter jejuni and C. coli and atypical C. lari strains, which shared identical or nearly identical 16S rDNA sequences. Subsequently, it was investigated whether partial 16S rDNA sequences are sufficient to determine species identity. Sequence alignments led to the identification of four 16S rDNA regions with high degrees of interspecies variation but with highly conserved sequence patterns within the respective species. A simple protocol based on the analysis of these sequence patterns was developed, which enabled the unambiguous identification of the majority of Campylobacter species. We recommend 16S rDNA sequence analysis as an effective, rapid procedure for the specific identification of campylobacters.

* Corresponding author. Mailing address: Institute of Molecular Biology, Biochemistry and Microbiology, Karl-Franzens University, Graz, Heinrichstr. 31a, A-8010 Graz, Austria. Phone: 43-316-380-1902. Fax: 43-316-380-9016. E-mail: gregor.gorkiewicz{at}uni-graz.at.

Journal of Clinical Microbiology, June 2003, p. 2537-2546, Vol. 41, No. 6
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.6.2537-2546.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Korczak, B. M., Zurfluh, M., Emler, S., Kuhn-Oertli, J., Kuhnert, P. (2009). Multiplex Strategy for Multilocus Sequence Typing, fla Typing, and Genetic Determination of Antimicrobial Resistance of Campylobacter jejuni and Campylobacter coli Isolates Collected in Switzerland. J. Clin. Microbiol. 47: 1996-2007 [Abstract] [Full Text]  
  • Wilson, D. J., Gabriel, E., Leatherbarrow, A. J.H., Cheesbrough, J., Gee, S., Bolton, E., Fox, A., Hart, C. A., Diggle, P. J., Fearnhead, P. (2009). Rapid Evolution and the Importance of Recombination to the Gastroenteric Pathogen Campylobacter jejuni. Mol Biol Evol 26: 385-397 [Abstract] [Full Text]  
  • Acke, E., McGill, K., Golden, O., Jones, B. R., Fanning, S., Whyte, P. (2009). Prevalence of thermophilic Campylobacter species in household cats and dogs in Ireland. Vet Rec. 164: 44-47 [Abstract] [Full Text]  
  • Poppert, S., Haas, M., Yildiz, T., Alter, T., Bartel, E., Fricke, U., Essig, A. (2008). Identification of Thermotolerant Campylobacter Species by Fluorescence In Situ Hybridization. J. Clin. Microbiol. 46: 2133-2136 [Abstract] [Full Text]  
  • Chhour, K.-L., Hinds, L. A., Deane, E. M., Jacques, N. A. (2008). The microbiome of the cloacal openings of the urogenital and anal tracts of the tammar wallaby, Macropus eugenii. Microbiology 154: 1535-1543 [Abstract] [Full Text]  
  • Kawasaki, S., Fratamico, P. M., Wesley, I. V., Kawamoto, S. (2008). Species-Specific Identification of Campylobacters by PCR-Restriction Fragment Length Polymorphism and PCR Targeting of the Gyrase B Gene. Appl. Environ. Microbiol. 74: 2529-2533 [Abstract] [Full Text]  
  • Abdelbaqi, K., Buissonniere, A., Prouzet-Mauleon, V., Gresser, J., Wesley, I., Megraud, F., Menard, A. (2007). Development of a Real-Time Fluorescence Resonance Energy Transfer PCR To Detect Arcobacter Species. J. Clin. Microbiol. 45: 3015-3021 [Abstract] [Full Text]  
  • Korczak, B. M., Stieber, R., Emler, S., Burnens, A. P., Frey, J., Kuhnert, P. (2006). Genetic relatedness within the genus Campylobacter inferred from rpoB sequences.. Int. J. Syst. Evol. Microbiol. 56: 937-945 [Abstract] [Full Text]  
  • Hill, J. E., Paccagnella, A., Law, K., Melito, P. L., Woodward, D. L., Price, L., Leung, A. H., Ng, L.-K., Hemmingsen, S. M., Goh, S. H. (2006). Identification of Campylobacter spp. and discrimination from Helicobacter and Arcobacter spp. by direct sequencing of PCR-amplified cpn60 sequences and comparison to cpnDB, a chaperonin reference sequence database.. J Med Microbiol 55: 393-399 [Abstract] [Full Text]  
  • Mazzeo, M. F., Sorrentino, A., Gaita, M., Cacace, G., Di Stasio, M., Facchiano, A., Comi, G., Malorni, A., Siciliano, R. A. (2006). Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry for the Discrimination of Food-Borne Microorganisms. Appl. Environ. Microbiol. 72: 1180-1189 [Abstract] [Full Text]  
  • van Bergen, M. A. P., Dingle, K. E., Maiden, M. C. J., Newell, D. G., van der Graaf-Van Bloois, L., van Putten, J. P. M., Wagenaar, J. A. (2005). Clonal Nature of Campylobacter fetus as Defined by Multilocus Sequence Typing. J. Clin. Microbiol. 43: 5888-5898 [Abstract] [Full Text]  
  • Tu, Z.-C., Eisner, W., Kreiswirth, B. N., Blaser, M. J. (2005). Genetic Divergence of Campylobacter fetus Strains of Mammal and Reptile Origins. J. Clin. Microbiol. 43: 3334-3340 [Abstract] [Full Text]  
  • Workman, S. N., Mathison, G. E., Lavoie, M. C. (2005). Pet Dogs and Chicken Meat as Reservoirs of Campylobacter spp. in Barbados. J. Clin. Microbiol. 43: 2642-2650 [Abstract] [Full Text]  
  • Han, X. Y., Tarrand, J. J., Rice, D. C. (2005). Oral Campylobacter Species Involved in Extraoral Abscess: a Report of Three Cases. J. Clin. Microbiol. 43: 2513-2515 [Abstract] [Full Text]  
  • Song, Y., Liu, C., Bolanos, M., Lee, J., McTeague, M., Finegold, S. M. (2005). Evaluation of 16S rRNA Sequencing and Reevaluation of a Short Biochemical Scheme for Identification of Clinically Significant Bacteroides Species. J. Clin. Microbiol. 43: 1531-1537 [Abstract] [Full Text]  
  • Klena, J. D., Parker, C. T., Knibb, K., Ibbitt, J. C., Devane, P. M. L., Horn, S. T., Miller, W. G., Konkel, M. E. (2004). Differentiation of Campylobacter coli, Campylobacter jejuni, Campylobacter lari, and Campylobacter upsaliensis by a Multiplex PCR Developed from the Nucleotide Sequence of the Lipid A Gene lpxA. J. Clin. Microbiol. 42: 5549-5557 [Abstract] [Full Text]  
  • Karenlampi, R. I., Tolvanen, T. P., Hanninen, M.-L. (2004). Phylogenetic Analysis and PCR-Restriction Fragment Length Polymorphism Identification of Campylobacter Species Based on Partial groEL Gene Sequences. J. Clin. Microbiol. 42: 5731-5738 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»