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Journal of Clinical Microbiology, June 2003, p. 2644-2646, Vol. 41, No. 6
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.6.2644-2646.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Use of Colony Morphology To Distinguish Different Enterococcal Strains and Species in Mixed Culture from Clinical Specimens

Shabnam Qamer,1 Jonathan A. T. Sandoe,1,2* and Kevin G. Kerr1,2,{dagger}

Department of Microbiology, University of Leeds,1 General Infirmary at Leeds, Leeds, United Kingdom2

Received 25 October 2002/ Returned for modification 19 January 2003/ Accepted 14 February 2003

Colony morphology on kanamycin esculin azide agar was investigated as a means of selecting different species and strains of enterococci from clinical specimens. Four representative colonies of each morphotype were indistinguishable by pulsed-field gel electrophoresis, biotype, and antibiogram analysis. The optimum time for identification of different colony morphotypes was 72 h.

* Corresponding author. Mailing address: Department of Microbiology, University of Leeds and General Infirmary at Leeds, Leeds, United Kingdom LS2 9JT. Phone: 00 44 113 343 5634. Fax: 00 44 113 343 5638. E-mail: j.sandoe{at}leeds.ac.uk.

{dagger} Present address: Harrogate District Hospital, Lancaster Park Road, Harrogate, United Kingdom.

Journal of Clinical Microbiology, June 2003, p. 2644-2646, Vol. 41, No. 6
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.6.2644-2646.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.





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