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Journal of Clinical Microbiology, June 2003, p. 2647-2649, Vol. 41, No. 6
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.6.2647-2649.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Utility of an In-House Mycobacteriophage-Based Assay for Rapid Detection of Rifampin Resistance in Mycobacterium tuberculosis Clinical Isolates

N. Galí, J. Domínguez,* S. Blanco, C. Prat, M. D. Quesada, L. Matas, and V. Ausina

Servei de Microbiologia, Hospital Universitari Germans Trias i Pujol, and Departament de Genètica i Microbiologia, Universitat Autònoma de Barcelona, Barcelona, Spain

Received 10 September 2002/ Returned for modification 16 October 2002/ Accepted 19 February 2003

A rapid in-house mycobacteriophage-based assay to identify multidrug resistance by detecting the rifampin susceptibility of Mycobacterium tuberculosis in a microtiter plate format was evaluated. The sensitivity, specificity, and overall accuracy of the assay were 100%. This test is rapid to perform and suitable for widespread implementation.

* Corresponding author. Mailing address: Servei de Microbiologia, Hospital Universitari Germans Trias i Pujol, Ctra. del Canyet, s/n 08916 Badalona, Barcelona, Spain. Phone: 34-93-497 88 94. Fax: 34-93-497 88 95. E-mail: jadoming{at}ns.hugtip.scs.es.

Journal of Clinical Microbiology, June 2003, p. 2647-2649, Vol. 41, No. 6
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.6.2647-2649.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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