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Journal of Clinical Microbiology, June 2003, p. 2662-2664, Vol. 41, No. 6
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.6.2662-2664.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Comparison of Washing and Swabbing Procedures for Collecting Genital Fluids To Assess Cervicovaginal Shedding of Herpes Simplex Virus Type 2 DNA

Laboratoire de Virologie, Département de Microbiologie, Université des Sciences de la Santé,¹ Maternité Joséphine Bongo,² Service de Gynécologie et Obstétrique, Centre Hospitalier de Libreville, Libreville, Gabon,⁵ Laboratoire de Virologie, Hôpital Européen Georges Pompidou, and Université Pierre et Marie Curie (Paris VI),³ Equipe Transmission Muqueuse du VIH et Immunité Locale, Unité INSERM 430, Institut Biologique des Cordeliers, Faculté de Médecine Broussais-Hôtel Dieu, Paris, France,⁶ Department of Oral Medicine, Eastman Institute, UCL, University of London, London, United Kingdom⁴

Received 17 June 2002/ Returned for modification 20 September 2002/ Accepted 28 February 2003

Asymptomatic genital shedding of herpes simplex virus type 2 (HSV-2) DNA was evidenced by real-time PCR in 25 (13.2%) of 188 cervicovaginal lavage samples and in only 13 (6.9%) paired cervicovaginal samples from 188 HSV-2-seropositive, nonpregnant childbearing-aged human immunodeficiency virus-seronegative women living in Gabon. These observations demonstrate that cervicovaginal washing is more suitable than endocervicovaginal swabbing for detecting and quantifying HSV-2 DNA by PCR in female genital secretions.

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