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Journal of Clinical Microbiology, June 2003, p. 2665-2668, Vol. 41, No. 6
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.6.2665-2668.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Comparison of Results Obtained by Testing with Three Different Agar Media and by the NCCLS M27-A Method for In Vitro Testing of Fluconazole against Candida spp.

M. Carmen Rubio,1* Joaquina Gil,1 Inmaculada Ramírez de Ocáriz,1 Rafael Benito,1 and Antonio Rezusta2

Microbiology, Hospital Clínico Universitario Lozano Blesa, Zaragoza,1 Facultad de Ciencias de la Salud y del Deporte, Huesca, Spain2

Received 12 November 2002/ Returned for modification 17 January 2003/ Accepted 21 March 2003

Fluconazole susceptibilities of 150 Candida isolates were determined by a 25-µg fluconazole disk diffusion agar test and compared with the microdilution NCCLS M27-A method. The agar test used three different media and was read at 24 and 48 h. When only the susceptible and nonsusceptible categories were used, disk diffusion with Müeller-Hinton agar supplemented with 2% glucose and 0.5 µg of methylene blue (MHGM) per ml had a 95.37% correlation with the MIC method at 24 h, followed by RPMI 1640-2% of glucose agar (correlation, 94%) and Shadomy medium (SHDM) (correlation, 92.6%). The growth of microcolonies inside the inhibition zones was common (>63%) in the RPMI and SHDM media and minimal with MHGM (8.7%). At 48 h, MHGM and SHDM still had a >91% correlation with the MIC, while RPMI results had dropped to 75%. The best overall agreement was obtained with C. dubliniensis (100%).

* Corresponding author. Mailing address: Servicio de Microbiologia, Hospital Clínico Universitario Lozano Blesa, Domingo Miral s/n, Zaragoza 50009, Spain. Phone: 34-976-556400, ext. 4322. Fax: 34-976-761693. E-mail: mcrubio{at}posta.unizar.es.

Journal of Clinical Microbiology, June 2003, p. 2665-2668, Vol. 41, No. 6
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.6.2665-2668.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Espinel-Ingroff, A., Canton, E., Gibbs, D., Wang, A. (2007). Correlation of Neo-Sensitabs Tablet Diffusion Assay Results on Three Different Agar Media with CLSI Broth Microdilution M27-A2 and Disk Diffusion M44-A Results for Testing Susceptibilities of Candida spp. and Cryptococcus neoformans to Amphotericin B, Caspofungin, Fluconazole, Itraconazole, and Voriconazole. J. Clin. Microbiol. 45: 858-864 [Abstract] [Full Text]  


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