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Journal of Clinical Microbiology, June 2003, p. 2676-2679, Vol. 41, No. 6
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.6.2676-2679.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Epitope-Blocking Enzyme-Linked Immunosorbent Assays for Detection of West Nile Virus Antibodies in Domestic Mammals

Bradley J. Blitvich,1 Richard A. Bowen,2 Nicole L. Marlenee,1 Roy A. Hall,3 Michel L. Bunning,4,5 and Barry J. Beaty1*

Arthropod-Borne and Infectious Diseases Laboratory, Department of Microbiology, Immunology and Pathology,1 Animal Reproduction and Biotechnology Laboratory, Equine Center, Colorado State University, Fort Collins, Colorado 80523,2 Department of Microbiology and Parasitology, The University of Queensland, St. Lucia, Queensland, Australia 4072,3 Division of Vector-Borne Infectious Diseases, Centers for Disease Control and Prevention, Fort Collins, Colorado 80522,4 Office of the Surgeon General, United States Air Force, Washington, D.C. 203325

Received 5 February 2003/ Returned for modification 16 March 2003/ Accepted 26 March 2003

We evaluated the ability of epitope-blocking enzyme-linked immunosorbent assays (ELISAs) to detect West Nile virus (WNV) antibodies in domestic mammals. Sera were collected from experimentally infected horses, cats, and pigs at regular intervals and screened in ELISAs and plaque reduction neutralization tests. The diagnostic efficacies of these techniques were similar.

* Corresponding author. Mailing address: Arthropod-Borne and Infectious Diseases Laboratory, Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO 80523. Phone: (970) 491-2988. Fax: (970) 491-8323. E-mail: bbeaty{at}colostate.edu.

Journal of Clinical Microbiology, June 2003, p. 2676-2679, Vol. 41, No. 6
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.6.2676-2679.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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Antimicrob. Agents Chemother. Clin. Microbiol. Rev.
Clin. Vaccine Immunol. ALL ASM JOURNALS

Copyright © 2003 by the American Society for Microbiology. All rights reserved.