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Journal of Clinical Microbiology, June 2003, p. 2683-2685, Vol. 41, No. 6
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.6.2683-2685.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Evaluation of the Epidemiologic Utility of Secondary Typing Methods for Differentiation of Mycobacterium tuberculosis Isolates

Awewura Kwara,1* Ronald Schiro,2 Lauren S. Cowan,3 Newton E. Hyslop,1 Mark F. Wiser,4 Stephanie Roahen Harrison,5 Patricia Kissinger,5 Lois Diem,3 and Jack T. Crawford3

Department of Tropical Medicine,4 Department of Epidemiology, Tulane University School of Public HealthTropical Medicine,5 Section of Adult Infectious Diseases, Tulane University Health Sciences Center,1 Office of Public Health, Louisiana Department of Health and Hospitals, New Orleans, Louisiana 70112,2 Division of AIDS, STD, and TB Laboratory Research, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 303333

Received 13 December 2002/ Returned for modification 13 February 2003/ Accepted 24 March 2003

Spoligotyping and mycobacterial interspersed repetitive unit-variable-number tandem repeat analysis (MIRU-VNTR) were evaluated for the ability to differentiate 64 Mycobacterium tuberculosis isolates from 10 IS6110-defined clusters. MIRU-VNTR performed slightly better than spoligotyping in reducing the number of clustered isolates and the sizes of the clusters. All epidemiologically related isolates remained clustered by MIRU-VNTR but not by spoligotyping.


* Corresponding author. Mailing address: Infectious Diseases Division, Miriam Hospital, 164 Summit Ave., Providence, RI 02906-0609. Phone: (401) 793-2463. Fax: (401) 793-4704. E-mail: Awewura_Kwara{at}brown.edu.


Journal of Clinical Microbiology, June 2003, p. 2683-2685, Vol. 41, No. 6
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.6.2683-2685.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.




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