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Journal of Clinical Microbiology, June 2003, p. 2690-2693, Vol. 41, No. 6
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.6.2690-2693.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Molecular Characterization of Isolates of Waterborne Cryptosporidium spp. Collected during an Outbreak of Gastroenteritis in South Burgundy, France

Frédéric Dalle,1 Pascale Roz,1 Guillaume Dautin,1 Marc Di-Palma,2 Evelyne Kohli,3 C. Sire-Bidault,4 Marie George Fleischmann,5 Anne Gallay,6 Sylvia Carbonel,2 Fabienne Bon,3 Claude Tillier,5 Pascal Beaudeau,6 and Alain Bonnin1*

Laboratoire de Parasitologie Mycologie et Laboratoire de Microbiologie Médicale et Moléculaire (EA 562), CHU et Faculté de Médecine,1 DRASS de Bourgogne, CIR Centre Est,2 Laboratoire de Virologie et Laboratoire de Microbiologie Médicale et Moléculaire (EA 562), CHU et Faculté de Pharmacie, Dijon,3 Laboratoire de Biologie, Centre Hospitalier de Chalon sur Saône, Chalon sur Saône,4 DDASS de Saône et Loire, Macon,5 Institut de Veille Sanitaire, Saint Maurice, France6

Received 25 November 2002/ Returned for modification 12 January 2003/ Accepted 28 February 2003

In September 2001, a waterborne outbreak of gastroenteritis occurred in eastern France. Of 31 fecal samples from symptomatic individuals, 19 tested positive for Cryptosporidium with two PCRs targeting the Hsp70 and the 18S rRNA genes of Cryptosporidium. Sequencing of the PCR fragments produced sequences identical to that of Cryptosporidium parvum genotype 1.

* Corresponding author. Mailing address: Laboratoire de Parasitologie Mycologie, Hôpital du Bocage, BP 77908, 21079 Dijon Cedex, France. Phone: 33 380 29 36 03. Fax: 33 380 29 32 80. E-mail: alain.bonnin{at}chu-dijon.fr.

Journal of Clinical Microbiology, June 2003, p. 2690-2693, Vol. 41, No. 6
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.6.2690-2693.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Cohen, S., Dalle, F., Gallay, A., Di Palma, M., Bonnin, A., Ward, H. D. (2006). Identification of Cpgp40/15 Type Ib as the Predominant Allele in Isolates of Cryptosporidium spp. from a Waterborne Outbreak of Gastroenteritis in South Burgundy, France. J. Clin. Microbiol. 44: 589-591 [Abstract] [Full Text]  
  • Coupe, S., Sarfati, C., Hamane, S., Derouin, F. (2005). Detection of Cryptosporidium and Identification to the Species Level by Nested PCR and Restriction Fragment Length Polymorphism. J. Clin. Microbiol. 43: 1017-1023 [Abstract] [Full Text]  
  • Traversa, D., Giangaspero, A., Molini, U., Iorio, R., Paoletti, B., Otranto, D., Giansante, C. (2004). Genotyping of Cryptosporidium Isolates from Chamelea gallina Clams in Italy. Appl. Environ. Microbiol. 70: 4367-4370 [Abstract] [Full Text]  


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