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Journal of Clinical Microbiology, June 2003, p. 2703-2705, Vol. 41, No. 6
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.6.2703-2705.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Comparison of Microscan Broth Microdilution, Synergy Quad Plate Agar Dilution, and Disk Diffusion Screening Methods for Detection of High-Level Aminoglycoside Resistance in Enterococcus Species

David R. Murdoch,1* Stanley Mirrett,1 Lizzie J. Harrell,1 Susan M. Donabedian,2 Marcus J. Zervos,2 and L. Barth Reller1

Clinical Microbiology Laboratory, Duke University Medical Center, Durham, North Carolina 27710,1 William Beaumont Hospital, Royal Oak, Michigan 480682

Received 24 September 2002/ Returned for modification 3 December 2002/ Accepted 25 March 2003

We compared the dried MicroScan microdilution panel, Synergy Quad plate agar dilution, and high-potency disk diffusion screening methods for the detection of high-level aminoglycoside resistance in 815 enterococcal bloodstream isolates. Agreement between the three methods was 99% when testing for high-level gentamicin resistance and 96% when testing for high-level streptomycin resistance.

* Corresponding author. Mailing address: Microbiology Unit, Canterbury Health Laboratories, P.O. Box 151, Christchurch, New Zealand. Phone: 64 3 364 1530. Fax: 64 364 0238. E-mail: david.murdoch{at}cdhb.govt.nz.

Journal of Clinical Microbiology, June 2003, p. 2703-2705, Vol. 41, No. 6
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.6.2703-2705.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.





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