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Journal of Clinical Microbiology, June 2003, p. 2734-2736, Vol. 41, No. 6
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.6.2734-2736.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Detection of Chlamydophila felis and Feline Herpesvirus by Multiplex Real-Time PCR Analysis

Chris Helps,* Nicky Reeves, Kathy Egan, Pam Howard, and Dave Harbour

Division of Molecular and Cellular Biology, Department of Clinical Veterinary Science, University of Bristol, Langford, Bristol BS40 5DU, United Kingdom

Received 1 March 2002/ Accepted 1 November 2002

Chlamydophila felis and feline herpesvirus (FHV) are pathogens commonly associated with feline respiratory and ocular disease. A real-time multiplex PCR assay was developed to allow detection of these organisms, together with feline 28S ribosomal DNA, in a single tube. Of 538 ocular swab samples tested, 123 were positive for FHV, 97 were positive for C. felis, and 16 were positive for both pathogens.

* Corresponding author. Mailing address: Division of Molecular and Cellular Biology, Department of Clinical Veterinary Science, University of Bristol, Langford House, Langford, Bristol BS40 5DU, United Kingdom. Phone: 44 117 928 9242. Fax: 44 117 928 9505. E-mail: c.r.helps{at}bristol.ac.uk.

Journal of Clinical Microbiology, June 2003, p. 2734-2736, Vol. 41, No. 6
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.6.2734-2736.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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