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Journal of Clinical Microbiology, June 2003, p. 2737-2740, Vol. 41, No. 6
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.6.2737-2740.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Genetic Diversity of Gallibacterium anatis Isolates from Different Chicken Flocks

Anders Miki Bojesen,1* Mia Torpdahl,2 Henrik Christensen,1 John Elmerdahl Olsen,1 and Magne Bisgaard1

Department of Veterinary Microbiology, The Royal Veterinary and Agricultural University, DK-1870 Frederiksberg C,1 The Danish Veterinary Institute, DK-1790 Copenhagen V,Denmark2

Received 10 June 2002/ Returned for modification 20 November 2002/ Accepted 4 March 2003

Amplified fragment length polymorphisms (AFLPs) were used to characterize the genotypic diversity of a total of 114 Gallibacterium anatis isolates originating from a reference collection representing 15 biovars from four countries and isolates obtained from tracheal and cloacal swab samples of chickens from an organic, egg-producing flock and a layer parent flock. A subset of strains was also characterized by pulsed-field gel electrophoresis and biotyping. The organic flock isolates were characterized by more than 94% genetic similarity, indicating that only a single clone was apparent in the flock. The layer parent flock isolates were grouped into two subclusters, each with similarity above 90%. One subcluster contained only tracheal isolates, while the other primarily included cloacal isolates. In conclusion, we show that AFLP analysis enables fingerprinting of G. anatis, which seems to have a clonal population structure within natural populations. There was further evidence of clonal lineages, which may have adapted to different sites within the same animal.

* Corresponding author. Mailing address: Department of Veterinary Microbiology, The Royal Veterinary and Agricultural University, Stigboejlen 4, DK-1870 Frederiksberg C, Denmark. Phone: 45 35282781. Fax: 45 35282757. E-mail: miki{at}kvl.dk.

Journal of Clinical Microbiology, June 2003, p. 2737-2740, Vol. 41, No. 6
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.6.2737-2740.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.





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