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Journal of Clinical Microbiology, June 2003, p. 2744-2747, Vol. 41, No. 6
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.6.2744-2747.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Subgenotype Analysis of Cryptosporidium Isolates from Humans, Cattle, and Zoo Ruminants in Portugal

Margarida Alves,1* Lihua Xiao,2 Irshad Sulaiman,2 Altaf A. Lal,2 Olga Matos,1 and Francisco Antunes1,3

Unidade de Protozoários Oportunistas/VIH e Outras Protozooses, Unidade de Parasitologia e Microbiologia Médicas (UPMM), Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa, 1349-008 Lisbon,1 Clínica Universitária de Doenças Infecciosas, Faculdade de Medicina (Hospital de Santa Maria), Universidade de Lisboa, 1600 Lisbon, Portugal,3 Division of Parasitic Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 303412

Received 4 November 2002/ Returned for modification 27 December 2002/ Accepted 25 February 2003

Cryptosporidium parvum and Cryptosporidium hominis isolates from human immunodeficiency virus-infected patients, cattle, and wild ruminants were characterized by PCR and DNA sequencing analysis of the 60-kDa glycoprotein gene. Seven alleles were identified, three corresponding to C. hominis and four corresponding to C. parvum. One new allele was found (IId), and one (IIb) had only been found in Portugal. Isolates from cattle and wild ruminants clustered in two alleles. In contrast, human isolates clustered in seven alleles, showing extensive allelic diversity.

* Corresponding author. Mailing address: Unidade de Protozoários Oportunistas/VIH e Outras Protozooses, Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa, Rua da Junqueira, 96, 1349-008 Lisbon, Portugal. Phone: 351 21 365 26 00. Fax: 351 21 363 21 05. E-mail: malves{at}ihmt.unl.pt.

Journal of Clinical Microbiology, June 2003, p. 2744-2747, Vol. 41, No. 6
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.6.2744-2747.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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